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AB77353

Anti-Mannosidase II/MAN2A1 antibody

4

(1 Review)

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(1 Publication)

Goat Polyclonal Mannosidase II/MAN2A1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MANA2, MAN2A1, Alpha-mannosidase 2, Golgi alpha-mannosidase II, Mannosidase alpha class 2A member 1, AMan II, Man II

2 Images
Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB77353)
  • WB

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Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB77353)

All lanes:

Western blot - Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 0.3 µg/mL

All lanes:

Human Placenta lysate (in RIPA buffer) at 35 µg

Predicted band size: 131 kDa

Observed band size: 150 kDa

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Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB77353)
  • WB

Unknown

Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB77353)

All lanes:

Western blot - Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 3 µg/mL

Lane 1:

MOCK-transfected HEK293 cell lysate

Lane 2:

HEK293 cell lysate overexpressing Mannosidase II/MAN2A1

Predicted band size: 131 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab77353 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannosidase II also known as MAN2A1 is an enzyme that plays a mechanical role in the processing of N-linked oligosaccharides. It performs this task by cleaving mannose residues from glycoproteins during the process of glycan maturation. MAN2A1 has a mass of about 133 kDa and is predominantly located in the Golgi apparatus an important cellular organelle involved in modifying sorting and packaging proteins. This enzyme shows expression in various types of tissues contributing to different levels of glycoprotein processing in the human body.
Biological function summary

Mannosidase II is involved in glycoprotein maturation and functions as an important component within the N-glycan biosynthesis pathway. It does not form a permanent complex with other enzymes but interacts with other glycosylation enzymes to ensure sequential processing. This enzyme helps in maintaining the consistency and specificity of glycoprotein folding and function impacting cellular communication and stability. Various cells across tissues utilize glycoproteins for different cellular processes illustrating MAN2A1's importance in molecular biology.

Pathways

MAN2A1 plays a significant role in the N-glycan biosynthesis and quality control pathway. This pathway involves a cascade of enzymatic actions facilitating proper protein folding and cellular signaling. Within these pathways other essential proteins such as alpha-mannosidase I and N-acetylglucosaminyltransferase I work in tandem with mannosidase II. MAN2A1 contributes to the precision of glycosylation alterations affecting signal transductions and cell-cell interactions important in various physiological processes.

MAN2A1 associates with congenital disorders of glycosylation and certain types of cancer including hepatocellular carcinoma. Improper function or expression of this enzyme can lead to aberrant glycosylation patterns contributing to these diseases. In cancer progression glycosylation changes influence cell adhesion and immune surveillance affecting tumor behavior and metastasis. MAN2A1 can indirectly interact with proteins like E-cadherin through altered glycosylation influencing cell-cell adhesion and cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
See full target information MAN2A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular physiology and biochemistry : internation 51:2309-2323 PubMed30537737

2018

The Transient Receptor Potential Channel, Vanilloid 5, Induces Chondrocyte Apoptosis via Ca2+ CaMKII-Dependent MAPK and Akt/ mTOR Pathways in a Rat Osteoarthritis Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yingliang Wei,Zhaofeng Jin,He Zhang,Shang Piao,Jinghan Lu,Lunhao Bai
View all publications
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Product promise

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