AB156014

Anti-Map17 antibody [EPR10372]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Map17 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

MAP17, PDZK1IP1, PDZK1-interacting protein 1, 17 kDa membrane-associated protein, Protein DD96

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

ab156014 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

ab156014 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

ab156014 showing +ve staining in Human urinary bladder transitional carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (AB156014)

ab156014 showing +ve staining in Human colonic adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Map17 antibody [EPR10372] (AB156014)

WB

Unknown

Western blot - Anti-Map17 antibody [EPR10372] (AB156014)

All lanes:

Western blot - Anti-Map17 antibody [EPR10372] (ab156014) at 1/1000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10372

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Map17 also known as PDZK1IP1 or MCFD2 functions as a membrane-associated protein with a mass of approximately 17 kDa. It localizes primarily in the plasma membrane but can also be found in other cellular compartments. This protein is expressed in various tissues with high levels observed in renal and gastrointestinal tract tissues. Its structural characteristic includes a PDZ-binding motif which suggests interactions with PDZ domain-containing proteins playing a role in facilitating membrane transport processes.

Biological function summary

The membrane-associated protein Map17 enhances ion conductivity across the cell membrane contributing to fluid transport and cellular homeostasis. It is not part of a documented protein complex but it interacts with scaffolding proteins influencing cellular architecture and function. Map17 also links with cellular redox states regulating the balance between cellular oxidants and antioxidants. Due to its role in ion transport and cellular balance Map17 impacts various physiological processes within the body.

Pathways

Research indicates Map17 involvement in oxidative stress and ion transport pathways. It plays a part in the sodium-potassium transport pathway through interaction with proteins like CFTR and sodium channels affecting fluid balance and kidney function. Furthermore its role in oxidative stress pathways implicates it in cellular responses to reactive oxygen species where Map17 influences proteins like Nrf2 an important regulator of antioxidant defenses. These pathways show how Map17 integrates into broader signaling networks that manage cellular environment and stress responses.

Map17 shows a connection with conditions such as cancer and chronic kidney disease. Research indicates its overexpression in various tumors where it may interact with the oncogene Ras to influence tumor growth and progression. In chronic kidney disease its role in ion transport could contribute to altered renal function with proteins like CFTR also involved in this disease context. Understanding Map17’s involvement in these diseases offers insights into potential therapeutic targets and novel treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Auxiliary protein of electrogenic Na(+)-coupled sugar symporter SLC5A2/SGLT2 and SLC5A1/SGLT1 (PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Essential for the transporter activity of SLC5A2/SGLT2 but not SLC5A1/SGLT1 (PubMed : 37217492).

See full target information PDZK1IP1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:601-619 PubMed39839187

2025

MAP17 is a Novel NASH Progression Biomarker Associated with Macrophage Infiltration, Immunotherapy Response, and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Huang,Jiatong Chen,Shenglu Liu,Xin Xiang,Yang Long,Peng Tan,Wenguang Fu

Biological procedures online 25:20 PubMed37403034

2023

LINC00853 contributes to tumor stemness of gastric cancer through FOXP3-mediated transcription of PDZK1IP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Hu,Maoyuan Zhao,Shuangyuan Hu,Qingsong Liu,Wenhao Liao,Lina Wan,Feng Wei,Fangting Su,Yu Guo,Jinhao Zeng

Journal of experimental & clinical cancer research : CR 42:40 PubMed36737832

2023

MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Yen Hsiao,Shang-Mei Weng,Jenn-Ren Hsiao,Yi-Ying Wu,Jia-En Wu,Chia-Hao Tung,Wan-Lin Shen,Shu-Fang Sun,Wen-Tsung Huang,Cheng-Yao Lin,Shang-Hung Chen,Tse-Ming Hong,Yuh-Ling Chen,Jang-Yang Chang

Frontiers in bioscience (Landmark edition) 26:777-788 PubMed34719205

2021

80MAP17 promotes the tumorigenesis of papillary thyroid carcinoma by reducing the stability of p53.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yu,Hongjiang Lu,Yanhong Chen,Ying Xin,Zhuo Tan,Qiong Yang

Acta biochimica et biophysica Sinica 53:1579-1589 PubMed34693452

2021

Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shishuai Wen,Yi Luo,Weili Wu,Tingting Zhang,Yichen Yang,Qinghai Ji,Yijun Wu,Rongliang Shi,Ben Ma,Midie Xu,Ning Qu

Journal of experimental & clinical cancer research : CR 40:121 PubMed33832535

2021

Hypoxia-dependent expression of MAP17 coordinates the Warburg effect to tumor growth in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Dong,Rongkun Li,Jiaofeng Wang,Yan Zhang,Jianfeng Yao,Shu-Heng Jiang,Xiaona Hu,Mingxuan Feng,Zhijun Bao

Molecular diagnosis & therapy 23:635-644 PubMed31273628

2019

Combined Fascin-1 and MAP17 Expression in Breast Cancer Identifies Patients with High Risk for Disease Recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterini Christina Tampaki,Athanasios Tampakis,Afroditi Nonni,Markus von Flüe,Efstratios Patsouris,Konstantinos Kontzoglou,Gregory Kouraklis

Oncotarget 8:92589-92603 PubMed29190940

2017

Elevation of MAP17 enhances the malignant behavior of cells via the Akt/mTOR pathway in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhuang Chen,Yan Liao,Yaqun Yu,Pengpeng Zhu,Jun Li,Liling Qin,Weijia Liao,Zhaoquan Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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