AB249253

Anti-Map17 antibody [EPR10372] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Map17 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAP17, PDZK1IP1, PDZK1-interacting protein 1, 17 kDa membrane-associated protein, Protein DD96

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

ab156014 showing +ve staining in Human urinary bladder transitional carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

ab156014 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

ab156014 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

ab156014 showing +ve staining in Human colonic adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

This data was developed using ab156014, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Map17 antibody [EPR10372] (<a href='/en-us/products/primary-antibodies/map17-antibody-epr10372-ab156014'>ab156014</a>) at 1/1000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa

false

Unconjugated

Anti-Map17 antibody [EPR10372]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10372

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249253 is the carrier-free version of ab156014.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Map17 also known as PDZK1IP1 or MCFD2 functions as a membrane-associated protein with a mass of approximately 17 kDa. It localizes primarily in the plasma membrane but can also be found in other cellular compartments. This protein is expressed in various tissues with high levels observed in renal and gastrointestinal tract tissues. Its structural characteristic includes a PDZ-binding motif which suggests interactions with PDZ domain-containing proteins playing a role in facilitating membrane transport processes.

Biological function summary

The membrane-associated protein Map17 enhances ion conductivity across the cell membrane contributing to fluid transport and cellular homeostasis. It is not part of a documented protein complex but it interacts with scaffolding proteins influencing cellular architecture and function. Map17 also links with cellular redox states regulating the balance between cellular oxidants and antioxidants. Due to its role in ion transport and cellular balance Map17 impacts various physiological processes within the body.

Pathways

Research indicates Map17 involvement in oxidative stress and ion transport pathways. It plays a part in the sodium-potassium transport pathway through interaction with proteins like CFTR and sodium channels affecting fluid balance and kidney function. Furthermore its role in oxidative stress pathways implicates it in cellular responses to reactive oxygen species where Map17 influences proteins like Nrf2 an important regulator of antioxidant defenses. These pathways show how Map17 integrates into broader signaling networks that manage cellular environment and stress responses.

Map17 shows a connection with conditions such as cancer and chronic kidney disease. Research indicates its overexpression in various tumors where it may interact with the oncogene Ras to influence tumor growth and progression. In chronic kidney disease its role in ion transport could contribute to altered renal function with proteins like CFTR also involved in this disease context. Understanding Map17’s involvement in these diseases offers insights into potential therapeutic targets and novel treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Auxiliary protein of electrogenic Na(+)-coupled sugar symporter SLC5A2/SGLT2 and SLC5A1/SGLT1 (PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Essential for the transporter activity of SLC5A2/SGLT2 but not SLC5A1/SGLT1 (PubMed : 37217492).

See full target information PDZK1IP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Map17 antibody [EPR10372] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Western blot - Anti-Map17 antibody [EPR10372] - BSA and Azide free (AB249253)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise