AB199540

Anti-Map17 antibody [EPR16827]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Map17 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAP17, PDZK1IP1, PDZK1-interacting protein 1, 17 kDa membrane-associated protein, Protein DD96

3 Images

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Western blot - Anti-Map17 antibody [EPR16827] (AB199540)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature PMID : 10951271.

Human fetal brain, Human fetal heart, Human fetal spleen represent negative controls for Map17.

All lanes:

Western blot - Anti-Map17 antibody [EPR16827] (ab199540) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 12 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Supplier Data

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (AB199540)

Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab199540 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal kidney whole cell lysate 10ug (Input). Lane 2 : ab199540 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

Predicted band size: 12 kDa

false

Supplier Data

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (AB199540)

Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/300 dilution. Western blot was performed from the immunoprecipitate using anti-Map17 (ab156014) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

Predicted band size: 12 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16827

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/120", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Map17 also known as PDZK1IP1 or MCFD2 functions as a membrane-associated protein with a mass of approximately 17 kDa. It localizes primarily in the plasma membrane but can also be found in other cellular compartments. This protein is expressed in various tissues with high levels observed in renal and gastrointestinal tract tissues. Its structural characteristic includes a PDZ-binding motif which suggests interactions with PDZ domain-containing proteins playing a role in facilitating membrane transport processes.

Biological function summary

The membrane-associated protein Map17 enhances ion conductivity across the cell membrane contributing to fluid transport and cellular homeostasis. It is not part of a documented protein complex but it interacts with scaffolding proteins influencing cellular architecture and function. Map17 also links with cellular redox states regulating the balance between cellular oxidants and antioxidants. Due to its role in ion transport and cellular balance Map17 impacts various physiological processes within the body.

Pathways

Research indicates Map17 involvement in oxidative stress and ion transport pathways. It plays a part in the sodium-potassium transport pathway through interaction with proteins like CFTR and sodium channels affecting fluid balance and kidney function. Furthermore its role in oxidative stress pathways implicates it in cellular responses to reactive oxygen species where Map17 influences proteins like Nrf2 an important regulator of antioxidant defenses. These pathways show how Map17 integrates into broader signaling networks that manage cellular environment and stress responses.

Map17 shows a connection with conditions such as cancer and chronic kidney disease. Research indicates its overexpression in various tumors where it may interact with the oncogene Ras to influence tumor growth and progression. In chronic kidney disease its role in ion transport could contribute to altered renal function with proteins like CFTR also involved in this disease context. Understanding Map17’s involvement in these diseases offers insights into potential therapeutic targets and novel treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Auxiliary protein of electrogenic Na(+)-coupled sugar symporter SLC5A2/SGLT2 and SLC5A1/SGLT1 (PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Essential for the transporter activity of SLC5A2/SGLT2 but not SLC5A1/SGLT1 (PubMed : 37217492).

See full target information PDZK1IP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Map17 antibody [EPR16827]

Western blot - Anti-Map17 antibody [EPR16827] (AB199540)

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (AB199540)

Immunoprecipitation - Anti-Map17 antibody [EPR16827] (AB199540)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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