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AB184350

Anti-MAP1A antibody [EPR18994]

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(3 Publications)

Rabbit Recombinant Monoclonal MAP1A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Mtap1, Mtap1a, Map1a, Microtubule-associated protein 1A, MAP-1A

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (AB184350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (AB184350)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (AB184350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (AB184350)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] (AB184350)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] (AB184350)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC)at 1/150 dilution was used as the secondary antibody.

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)
  • WB

Supplier Data

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAP1A antibody [EPR18994] (ab184350) at 1/2000 dilution

All lanes:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 305 kDa

Observed band size: 300 kDa

false

Exposure time: 3min

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)
  • WB

Supplier Data

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAP1A antibody [EPR18994] (ab184350) at 1/5000 dilution

Lane 1:

Mouse embryo lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

Mouse spinal cord lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 305 kDa

Observed band size: 26 kDa,300 kDa

false

Exposure time: 15s

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)
  • WB

Supplier Data

Western blot - Anti-MAP1A antibody [EPR18994] (AB184350)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAP1A antibody [EPR18994] (ab184350) at 1/1000 dilution

All lanes:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 305 kDa

Observed band size: 26 kDa,300 kDa

false

Exposure time: 30s

  • Carrier free

    Anti-MAP1A antibody [EPR18994] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18994

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
See full target information Map1a
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:310 PubMed40128722

2025

Electroacupuncture attenuates intervertebral disc degeneration by upregulating aquaporins via the cAMP/PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Jia-Bao Huang,Jing Zou,Guo-Fu Huang

Science advances 7: PubMed33741591

2021

Persistent repression of tau in the brain using engineered zinc finger protein transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Wegmann,Sarah L DeVos,Bryan Zeitler,Kimberly Marlen,Rachel E Bennett,Marta Perez-Rando,Danny MacKenzie,Qi Yu,Caitlin Commins,Riley N Bannon,Bianca T Corjuc,Alison Chase,Lisa Diez,Hoang-Oanh B Nguyen,Sarah Hinkley,Lei Zhang,Alicia Goodwin,Annemarie Ledeboer,Stephen Lam,Irina Ankoudinova,Hung Tran,Nicholas Scarlott,Rainier Amora,Richard Surosky,Jeffrey C Miller,Ashley B Robbins,Edward J Rebar,Fyodor D Urnov,Michael C Holmes,Amy M Pooler,Brigit Riley,H Steve Zhang,Bradley T Hyman

Journal of neuroscience research 96:1798-1815 PubMed30054946

2018

Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention.

Applications

Unspecified application

Species

Unspecified reactive species

Javed Iqbal,Kaoyuan Zhang,Na Jin,Yuxi Zhao,Qiong Liu,Jiazuan Ni,Liming Shen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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