Rabbit Recombinant Monoclonal MAP1A antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--immunocytochemistry-immunofluorescence-img122940.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151)")
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--immunocytochemistry-immunofluorescence-img69469.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151)")
![Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--flow-cytometry-intracellular-img80722.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (AB223151)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
Alternative names
Microtubule-associated protein 1A, MAP-1A, Map1a, Mtap1, Mtap1a
Recommended products
Rabbit Recombinant Monoclonal MAP1A antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples.
Alternative names
Microtubule-associated protein 1A, MAP-1A, Map1a, Mtap1, Mtap1a
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18994
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab223151 is the carrier-free version of Anti-MAP1A antibody [EPR18994] ab184350.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--immunocytochemistry-immunofluorescence-img122940.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)")
Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with Anti-MAP1A antibody [EPR18994] ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-MAP1A antibody [EPR18994] ab184350 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MAP1A antibody [EPR18994] ab184350).
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--immunocytochemistry-immunofluorescence-img69469.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)")
Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling MAP1A with Anti-MAP1A antibody [EPR18994] ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-MAP1A antibody [EPR18994] ab184350 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MAP1A antibody [EPR18994] ab184350).
![Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151), expandable thumbnail](https://content.abcam.com/products/images/map1a-antibody-epr18994-bsa-and-azide-free-ab223151--flow-cytometry-intracellular-img80722.jpg "Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)")
Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with Anti-MAP1A antibody [EPR18994] ab184350 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MAP1A antibody [EPR18994] ab184350).
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