AB224115

Anti-MAP1B antibody

Name: Anti-MAP1B antibody (ab224115) | Abcam
Brand: Abcam Limited
SKU: AB224115

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(3 Publications)

Rabbit Polyclonal MAP1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP1B aa 1300-1500.

View Alternative Names

Microtubule-associated protein 1B, MAP-1B, MAP1B

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in seminiferous ducts at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemical analysis of human cerebral cortex tissue labeling MAP1B in the cytoplasm of neurones at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemical analysis of human skeletal muscle tissue labeling MAP1B in mytocytes (low positivity) at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody (AB224115)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody (AB224115)

Immunocytochemical analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling MAP1B in the cytosol at 2 μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in granular layer at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MAP1B antibody (AB224115)

Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-MAP1B antibody (ab224115) at 0.4 µg/mL

Lane 1:

Control siRNA transfected - U-251 MG (human brain glioma cell line) whole cell lysate

Lane 2:

siRNA probe#1 transfected - U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

siRNA probe#2 transfected - U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 271 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAP1B aa 1300-1500. The exact immunogen used to generate this antibody is proprietary information.

P46821

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B is required for proper microtubule dynamics and plays a role in the cytoskeletal changes that accompany neuronal differentiation and neurite extension (PubMed : 33268592). Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.

See full target information MAP1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 11:1240039 PubMed37691832

2023

Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Bilekova,Balma Garcia-Colomer,Alberto Cebrian-Serrano,Silvia Schirge,Karsten Krey,Michael Sterr,Thomas Kurth,Stefanie M Hauck,Heiko Lickert

Stem cells international 2023:2826815 PubMed37964983

2023

A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin Liu

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP1B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody (AB224115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (AB224115)

Western blot - Anti-MAP1B antibody (AB224115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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