Rabbit Polyclonal MAP1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP1B aa 1300-1500.
Images
Publications
Filter by
- Frontiers in cell and developmental biology 11:12400392023Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Bilekova,Balma Garcia-Colomer,Alberto Cebrian-Serrano,Silvia Schirge,Karsten Krey,Michael Sterr,Thomas Kurth,Stefanie M Hauck,Heiko LickertPubMed 37691832
- Cell genomics 3:1002502023Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper LagePubMed 36950384
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAP1B aa 1300-1500. The exact immunogen used to generate this antibody is proprietary information. Database link P46821
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B is required for proper microtubule dynamics and plays a role in the cytoskeletal changes that accompany neuronal differentiation and neurite extension (PubMed:33268592). Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
Alternative names
Microtubule-associated protein 1B, MAP-1B, MAP1B
Recommended products
Rabbit Polyclonal MAP1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP1B aa 1300-1500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAP1B aa 1300-1500. The exact immunogen used to generate this antibody is proprietary information. Database link P46821
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-MAP1B antibody (ab224115)
Loading control: Anti-GAPDH.
All lanes: Western blot - Anti-MAP1B antibody (ab224115) at 0.4 µg/mL
Lane 1: Control siRNA transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2: siRNA probe#1 transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3: siRNA probe#2 transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 271 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)
Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in seminiferous ducts at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody (ab224115)
Immunocytochemical analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling MAP1B in the cytosol at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)
Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in granular layer at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)
Immunohistochemical analysis of human cerebral cortex tissue labeling MAP1B in the cytoplasm of neurones at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)
Immunohistochemical analysis of human skeletal muscle tissue labeling MAP1B in mytocytes (low positivity) at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com