Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neuron of human cerebrum (PMID : 25429138; PMID : 24614691). The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human glomeruli (PMID : 26448484). The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human skeletal muscle. The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. MAP1B was immunoprecipitated from 0.35 mg human cerebellum tissue lysate 10 ug with ab307286 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307286 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human cerebellum tissue lysate 10 ug Lane 2 : ab307286 IP in human cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307286 in human cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] (<a href='/en-us/products/primary-antibodies/map1b-antibody-epr27225-25-ab307286'>ab307286</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate 10 μg

Lane 2:

Human cerebellum tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 26 kDa,32 kDa

false

Exposure time: 10s

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum (PMID : 25788676). The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on rat skeletal muscle. The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labeling MAP1B with ab307286 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling MAP1B with ab307286 at 1/100 (4.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cerebellum. The section was incubated with ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labeling MAP1B with ab307286 at 1/100 (4.96 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (2ug/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307286 at 1/100 dilution followed by ab150120 at a 1/1000 dilution. -ve control 2 : ab11267 at 1/500 dilution followed by ab150081 at a 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling MAP1B with ab307286 at 1/100 (4.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cerebellum. The section was incubated with ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebellum (PMID : 25788676). The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAP1B with ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on mouse skeletal muscle. The section was incubated with ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling MAP1B with ab307286 at 1/100 (4.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse skeletal muscle. The section was incubated with ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling MAP1B with ab307286 at 1/100 (4.96 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on rat skeletal muscle. The section was incubated with ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. MAP1B was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 ug with ab307286 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307286 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse cerebellum tissue lysate 10 ug Lane 2 : ab307286 IP in mouse cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307286 in mouse cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] (<a href='/en-us/products/primary-antibodies/map1b-antibody-epr27225-25-ab307286'>ab307286</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate 10 μg

Lane 2:

Mouse cerebellum tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 26 kDa,32 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Blocking/dilution buffer : 5% NFDM/TBST. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18656471; PMID : 16938900). Negative control : skeletal muscle tissue (PMID : 12684070). Exposure time : 180 seconds.

All lanes:

Western blot - Anti-MAP1B antibody [EPR27225-25] (<a href='/en-us/products/primary-antibodies/map1b-antibody-epr27225-25-ab307286'>ab307286</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate 20 μg

Lane 2:

Human skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,32 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (AB307287)

This data was developed using ab307286, the same antibody clone in a different buffer formulation. Blocking/dilution buffer : 5% NFDM/TBST. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18656471; PMID : 16938900). Negative control : skeletal muscle tissue (PMID : 12684070). Exposure time : 15 seconds.

All lanes:

Western blot - Anti-MAP1B antibody [EPR27225-25] (<a href='/en-us/products/primary-antibodies/map1b-antibody-epr27225-25-ab307286'>ab307286</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate 20 μg

Lane 2:

Mouse skeletal muscle tissue lysate 20 μg

Lane 3:

Rat cerebellum tissue lysate 20 μg

Lane 4:

Rat skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,32 kDa

false

Exposure time: 15s