Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free

17 product images

• 

  Western blot - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Blocking/dilution buffer: 5% NFDM/TBST.
  
  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18656471; PMID: 16938900).
  
  Negative control: skeletal muscle tissue (PMID: 12684070).
  
  Exposure time: 180 seconds.

  All lanes: Western blot - Anti-MAP1B antibody [EPR27225-25] (Anti-MAP1B antibody [EPR27225-25] ab307286) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate 20 μg

  Lane 2: Human skeletal muscle tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 26 kDa, 32 kDa

  Exposure time: 180s

• 

  Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  MAP1B was immunoprecipitated from 0.35 mg human cerebellum tissue lysate 10 ug with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: Human cerebellum tissue lysate 10 ug
  
  Lane 2: Anti-MAP1B antibody [EPR27225-25] ab307286 IP in human cerebellum tissue lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MAP1B antibody [EPR27225-25] ab307286 in human cerebellum tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] (Anti-MAP1B antibody [EPR27225-25] ab307286) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate 10 μg

  Lane 2: Human cerebellum tissue lysate

  Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 26 kDa, 32 kDa

  Exposure time: 10s

• 

  Western blot - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Blocking/dilution buffer: 5% NFDM/TBST.
  
  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18656471; PMID: 16938900).
  
  Negative control: skeletal muscle tissue (PMID:12684070).
  
  Exposure time: 15 seconds.

  All lanes: Western blot - Anti-MAP1B antibody [EPR27225-25] (Anti-MAP1B antibody [EPR27225-25] ab307286) at 1/1000 dilution

  Lane 1: Mouse cerebellum tissue lysate 20 μg

  Lane 2: Mouse skeletal muscle tissue lysate 20 μg

  Lane 3: Rat cerebellum tissue lysate 20 μg

  Lane 4: Rat skeletal muscle tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 26 kDa, 32 kDa

  Exposure time: 15s

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Negative control: no staining on rat skeletal muscle.
  
  The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human glomeruli (PMID: 26448484). The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  MAP1B was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 ug with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: Mouse cerebellum tissue lysate 10 ug
  
  Lane 2: Anti-MAP1B antibody [EPR27225-25] ab307286 IP in mouse cerebellum tissue lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MAP1B antibody [EPR27225-25] ab307286 in mouse cerebellum tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-MAP1B antibody [EPR27225-25] (Anti-MAP1B antibody [EPR27225-25] ab307286) at 1/1000 dilution

  Lane 1: Mouse cerebellum tissue lysate 10 μg

  Lane 2: Mouse cerebellum tissue lysate

  Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 26 kDa, 32 kDa

  Exposure time: 10s

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebellum (PMID: 25788676). The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebellum (PMID: 25788676). The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Negative control: no staining on mouse skeletal muscle.
  
  The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neuron of human cerebrum (PMID: 25429138; PMID: 24614691). The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/1000 (0.496 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Negative control: no staining on human skeletal muscle.
  
  The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 (4.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cerebellum. The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 (4.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
  
  Confocal image showing cytoplasmic staining in mouse primary neuron.
  
  Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  
  Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (2ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
  
  -ve control 1: Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.
  
  -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 (4.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
  
  Negative control: confocal image showing no staining on rat skeletal muscle.
  
  The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Flow Cytometry (Intracellular) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
  
  A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 (4.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cerebellum. The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-MAP1B antibody [EPR27225-25] - BSA and Azide free (ab307287)

  This data was developed using Anti-MAP1B antibody [EPR27225-25] ab307286, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling MAP1B with Anti-MAP1B antibody [EPR27225-25] ab307286 at 1/100 (4.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
  
  Negative control: confocal image showing no staining on mouse skeletal muscle.
  
  The section was incubated with Anti-MAP1B antibody [EPR27225-25] ab307286 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.