Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker (AB244210)

Flow cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells of MAP1LC3A with ab244210.
Fixing / permeabilization of cells : Absolute methanol / 0.1% Tween-20 in PBS.
Primary antibody : ab244210 at 2 μg per ~10⁶ cells for 15 minutes at room temperature.
Secondary antibody : Goat anti-mouse PE labeled secondary antibody (1/1000) with incubation for 15 minutes in dark at room temperature.
Non-specific Control IgG, clone X63 was used under same conditions as negative control (black dashed).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker (AB244210)

Immunofluorescence analysis of MAP1LC3A expression in SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker (AB244210)

Immunofluorescence analysis of MAP1LC3A expression in C6 (rat glial tumor cell line) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

• WB

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Western blot - Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker (AB244210)

SDS-PAGE : 4-20% Bis-Tris denatured.

All lanes:

Western blot - Anti-MAP1LC3A antibody [BS405] - Autophagosome Marker (ab244210) at 10 µg/mL

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg

Secondary

All lanes:

Anti-mouse-HRP, 2 hrs at RT at 1/10000 dilution

Predicted band size: 14 kDa

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