AB52628

Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker

Name: Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (ab52628) | Abcam
Brand: Abcam Limited
SKU: AB52628
Price: 225 USD
Availability: InStock
Rating: 5 (8 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

(8 Reviews)

(18 Publications)

Rabbit Recombinant Monoclonal Microtubule-associated proteins 1A/1B light chain 3A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 18 publications.

View Alternative Names

Microtubule-associated protein 1 light chain 3 alpha, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, Microtubule-associated proteins 1A/1B light chain 3A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1LC3A

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling MAP1LC3A with purified ab52628 at 1/100 dilution (10 μg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

All lanes:

Western blot - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (ab52628) at 1/50000 dilution

All lanes:

Fetal brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 16 kDa

false

Carrier free

Anti-MAP1LC3A antibody [EP1528Y] - BSA and Azide free
HRP

HRP Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1528Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20713600, PubMed : 24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538).

See full target information MAP1LC3A

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

Molecular psychiatry 28:4622-4631 PubMed37723283

2023

Mitochondrial health index correlates with plasma circulating cell-free mitochondrial DNA in bipolar disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Rafaela C Cordeiro,Camila N C Lima,Gabriel R Fries,Giovana Zunta-Soares,Jair C Soares,João Quevedo,Giselli Scaini

International journal of molecular sciences 24: PubMed36769070

2023

The Activation of Reticulophagy by ER Stress through the ATF4-MAP1LC3A-CCPG1 Pathway in Ovarian Granulosa Cells Is Linked to Apoptosis and Necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huiduo Li,Yanan Jing,Xiaoya Qu,Jinyi Yang,Pengge Pan,Xinrui Liu,Hui Gao,Xiuying Pei,Cheng Zhang,Yanzhou Yang

British journal of pharmacology 178:2931-2947 PubMed33786819

2021

Oxoglaucine mediates Ca influx and activates autophagy to alleviate osteoarthritis through the TRPV5/calmodulin/CAMK-II pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Zhong,Huiping Long,Fei Chen,Yin Yu

Oncology letters 20:53-60 PubMed32565933

2020

Chloroquine increases the anti-cancer activity of epirubicin in A549 lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Ling Liang,Jing Zhang,Shen-Lin Du,Bin Zhang,Xuan Ma,Cui-Yun Wu,Yong-Jun Liu

The Journal of allergy and clinical immunology 146:1210-1213.e9 PubMed32272130

2020

The regulatory activity of autophagy in conjunctival fibroblasts and its possible role in vernal keratoconjunctivitis.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Brun,Elena Tarricone,Antonino Di Stefano,Elena Mattiuzzo,Parvaneh Mehrbod,Saeid Ghavami,Andrea Leonardi

Drug design, development and therapy 14:395-405 PubMed32099325

2020

Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MingBo Wei,YanLi Wu,Hui Liu,Chun Xie

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 44:1291-1299 PubMed30575805

2018

TSPO upregulation in bipolar disorder and concomitant downregulation of mitophagic proteins and NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Giselli Scaini,Tatiana Barichello,Gabriel R Fries,Elizabeth A Kennon,Taylor Andrews,Bobby R Nix,Giovana Zunta-Soares,Samira S Valvassori,Jair C Soares,João Quevedo

The Prostate 78:95-103 PubMed29134671

2017

Macrophage roles in the clearance of apoptotic cells and control of inflammation in the prostate gland after castration.

Applications

Unspecified application

Species

Unspecified reactive species

Juliete A F Silva,Alexandre Bruni-Cardoso,Taize M Augusto,Danilo M Damas-Souza,Guilherme O Barbosa,Sérgio L Felisbino,Dagmar R Stach-Machado,Hernandes F Carvalho

Oncotarget 8:104347-104358 PubMed29262645

2017

Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

April B Cabang,Keya De Mukhopadhyay,Sarah Meyers,Jay Morris,Paul V Zimba,Michael J Wargovich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker

Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

Western blot - Anti-MAP1LC3A antibody [EP1528Y] - Autophagosome Marker (AB52628)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (18)

Product promise