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Rabbit Recombinant Monoclonal Microtubule-associated proteins 1A/1B light chain 3A antibody. Autophagosome marker. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 15 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (AB52768), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (AB52768), expandable thumbnail
  • Western blot - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (AB52768), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (AB52768), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (AB52768), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Mouse
Predicted
Expected
Predicted
Predicted
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000 - 1/10000
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100 - 1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/40 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).

Alternative names

Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A

Recommended products

Rabbit Recombinant Monoclonal Microtubule-associated proteins 1A/1B light chain 3A antibody. Autophagosome marker. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1983Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768), expandable thumbnail
    Image from Buckingham EM et al. Biotechniques. 2014;57(5):241-4. Fig 1.; doi: 10.2144/000114226.

    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)

    Immunocytochemistry/Immunofluorescence analysis of human keratinocytes (pannel A) and VZV-infected melanoma cells (panel E) labelling MAP1LC3A with ab52768.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)

    Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) labelling MAP1LC3A with purified ab52768 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).

  • Western blot - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768), expandable thumbnail

    Western blot - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)

    All lanes: Western blot - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768) at 1/10000 dilution

    All lanes: Hela cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 14 kDa

    Observed band size: 16 kDa

  • Flow Cytometry (Intracellular) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)

    Overlay histogram showing SHSY-5Y cells stained with ab52768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52768, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768)

    Immunohistochemical staining of MAP1LC3A in paraffin embedded human breast carcinoma using ab52768 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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