Anti-MAP2 antibody [2C4] - Neuronal Marker

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody [2C4] - Neuronal Marker (AB315201)

Immunofluorescent analysis of adult rat hippocampus section stained with ab315201, dilution 1 : 5,000 in green, and chicken pAb to FOX2, CPCA-FOX2, dilution 1 : 2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. ab315201 antibody labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites. The FOX2 antibody stains the nuclei of most neuronal cells.

• WB

Supplier Data

Western blot - Anti-MAP2 antibody [2C4] - Neuronal Marker (AB315201)

Western blot analysis of tissue and cell lysates using ab315201, dilution 1 : 5,000 in green, and chicken pAb to vimentin, CPCA-Vim, dilution 1 : 5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293 and [6] NIH-3T3 cell lysates. A band at about 280kDa is full length intact MAP2A/2B isotypes while bands at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. The CPCA-Vim antibody binds to a single band at ~50kDa present in all preparations.

All lanes:

Western blot - Anti-MAP2 antibody [2C4] - Neuronal Marker (ab315201) at 1/5000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

HeLa cell lysate

Lane 4:

SH-SY5Y cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

NIH-3T3 cell lysate

Observed band size: 70 kDa,280 kDa

false