Anti-MAP2 antibody is a chicken polyclonal antibody that is used in MAP2 immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and western blot (WB). Suitable for mouse and rat samples.
- Cited in over 785 publications
- Tried and trusted by researchers since 2003
- Recombinant fragment protein within human MAP2 as immunogen
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- Aging and disease 15:1952-19682024Schizophrenia-Like Behaviors Arising from Dysregulated Proline Metabolism Are Associated with Altered Neuronal Morphology and Function in Mice with Hippocampal PRODH Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxiao Yao,Chenchen Jin,Yilie Liao,Xiang Huang,Ziying Wei,Yahong Zhang,Dongwei Li,Huanxing Su,Weiping Han,Dajiang QinPubMed 37815900
- Nature communications 14:84202023Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin ChanPubMed 38110419
- Cellular and molecular neurobiology 44:42023Mutation in the TRKB Cholesterol Recognition Site that blocks Antidepressant Binding does not Influence the Basal or BDNF-Stimulated Activation of TRKB.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline Biojone,Cecilia Cannarozzo,Nina Seiffert,Cassiano R A F Diniz,Cecilia A Brunello,Eero Castrén,Plinio CasarottoPubMed 38104054
- Nature communications 14:83542023Prion protein conversion at two distinct cellular sites precedes fibrillisation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Manuel Ribes,Mitali P Patel,Hazim A Halim,Antonio Berretta,Sharon A Tooze,Peter-Christian KlöhnPubMed 38102121
- Nature communications 14:82012023Immunoproximity biotinylation reveals the axon initial segment proteome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Yu Fu,Luxin Peng,Yuki Ogawa,Xiaoyun Ding,Anne Rasband,Xinyue Zhou,Maya Shelly,Matthew N Rasband,Peng ZouPubMed 38081810
- Communications biology 6:12112023Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc FerrerPubMed 38017066
- Communications biology 6:12092023Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Grochow,Britta Beck,Zaida Rentería-Solís,Gereon Schares,Pavlo Maksimov,Christina Strube,Lisa Raqué,Johannes Kacza,Arwid Daugschies,Simone A FietzPubMed 38012384
- Acta neuropathologica communications 11:1822023Neuronal dysfunction caused by FUSR521G promotes ALS-associated phenotypes that are attenuated by NF-κB inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMari Carmen Pelaez,Antoine Desmeules,Pauline A Gelon,Bastien Glasson,Laetitia Marcadet,Alicia Rodgers,Daniel Phaneuf,Silvia Pozzi,Paul A Dutchak,Jean-Pierre Julien,Chantelle F SephtonPubMed 37974279
- Cells 12:2023Treating Parkinson's Disease with Human Bone Marrow Mesenchymal Stem Cell Secretome: A Translational Investigation Using Human Brain Organoids and Different Routes of In Vivo Administration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Mendes-Pinheiro,Jonas Campos,Ana Marote,Carina Soares-Cunha,Sarah L Nickels,Anna S Monzel,Jorge R Cibrão,Eduardo Loureiro-Campos,Sofia C Serra,Sandra Barata-Antunes,Sara Duarte-Silva,Luísa Pinto,Jens C Schwamborn,António J SalgadoPubMed 37947643
- Science advances 9:eadf13322023Human cerebrospinal fluid affects chemoradiotherapy sensitivities in tumor cells from patients with glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrett W Stringer,Manam Inushi De Silva,Zarina Greenberg,Alejandra Noreña Puerta,Robert Adams,Bridget Milky,Michael Zabolocki,Mark van den Hurk,Lisa M Ebert,Christine Fairly Bishop,Simon J Conn,Ganessan Kichenadasse,Michael Z Michael,Rebecca J Ormsby,Santosh Poonoose,Cedric BardyPubMed 37878712
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.032% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAP2. The exact immunogen used to generate this antibody is proprietary information. Database link P11137
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Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Mouse | Expected | Expected | Tested |
| Rat | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/5000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50000 | Notes This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
Species Rat | Dilution info 1/20000 - 1/100000 | Notes This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
Associated Products
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Target data
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Alternative names
Microtubule-associated protein 2, MAP-2, MAP2
Recommended products
Anti-MAP2 antibody is a chicken polyclonal antibody that is used in MAP2 immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and western blot (WB). Suitable for mouse and rat samples.
- Cited in over 785 publications
- Tried and trusted by researchers since 2003
- Recombinant fragment protein within human MAP2 as immunogen
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.032% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAP2. The exact immunogen used to generate this antibody is proprietary information. Database link P11137
- Concentration
- Purification notes
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-MAP2 antibody (ab5392) is a chicken polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-MAP2 antibody (ab5392) was first used in a scientific publication in 2006 and has been cited over 788 times in peer reviewed journals. It's performance in IHC and Immunofluorescence in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-MAP2 antibody (ab5392) has high sensitivity and specificity.
Anti-MAP2 antibody (ab5392) has 62 independent reviews from customers.
Anti-MAP2 antibody (ab5392) specifically detects MAP2 (UniProt ID: P11137; Molecular weight: 200kDa) and is sold in 25 µL selling sizes.
MAP2, a dendritic marker, plays a crucial role in Alzheimer's disease (AD) research. Researchers use MAP2 to assess dendritic integrity in AD models, making it essential for understanding neuronal structure and pathology in Alzheimer's disease.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAP2 also known as Microtubule-associated protein 2 is a critical component in cellular mechanics. It aids in the stabilization and assembly of microtubules essential for maintaining the cytoskeleton structure. MAP2 has a molecular mass of approximately 280 kDa. It is highly expressed in the dendrites of neurons in the central nervous system. This expression pattern underlines its importance in dendritic development and maintenance. Though largely neuron-specific minor expression occurs in other tissues but with significantly less intensity.
- Biological function summary
MAP2 plays a role in maintaining neuronal structure. It participates in the formation of the cytoskeleton by binding to tubulin subunits in microtubules. By doing so it supports the outgrowth of dendrites critical for synaptic connections and neuronal communication. MAP2 does not typically form part of a complex but associates dynamically with other microtubule proteins to regulate microtubule dynamics. Its role extends to influencing the morphology of neurons which impacts information processing and neurotransmission.
- Pathways
MAP2 factors significantly into the regulation of the MAP kinase signaling pathway among others involving cytoskeletal remodeling. It interfaces with pathways controlled by proteins like MAP kinase affecting downstream targets involved in cellular growth and differentiation. MAP2 also interacts with tau proteins both being integral microtubule-associated proteins fostering structural support and transport within neurons.
- Associated diseases and disorders
MAP2 has a connection to neurodegenerative diseases such as Alzheimer's. Abnormal phosphorylation of MAP2 like that observed in tau leads to defective microtubule stability implicating it in neuronal dysfunction associated with Alzheimer's. Additionally some evidence points to MAP2's involvement in schizophrenia where altered dendritic morphology parallels changes in MAP2 expression. Its interaction with other microtubule-associated proteins especially tau further supports its relevance in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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9 product images
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)
MAP2 immunofluorescence staining of rat cortical neurons and glia using chicken anti-MAP2 antibody
Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
Immunocytochemistry - Anti-MAP2 antibody (ab5392)
MAP2 immunofluorescence staining of mouse neurons using chicken anti-MAP2 antibody
ab5392 at a dilution of 1/10000 staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
Western blot - Anti-MAP2 antibody (ab5392)
All lanes: Western blot - Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1: Adult rat brain lysate
Lane 2: Embryonic E20 rat brain lysate
Lane 3: Adult mouse brain lysate
Predicted band size: 199 kDa
Immunocytochemistry - Anti-MAP2 antibody (ab5392)
MAP2 immunofluorescence staining of rat cortical neurons and glia using chicken anti-MAP2 antibody
Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000) a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)
MAP2 immunofluorescence staining of rat cortical neurons and glia using chicken anti-MAP2 antibody
ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY diluted 1/400 was used as the secondary antibody.
This image is courtesy of an anonymous customer reviewWestern blot - Anti-MAP2 antibody (ab5392)
All lanes: Western blot - Anti-MAP2 antibody (ab5392) at 1/10000 dilution
All lanes: Mouse brain lysate - post nuclear supernatant at 10 µg
SecondaryAll lanes: HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 199 kDa
Observed band size: 280 kDa, 70 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
MAP2 immunohistochemistry staining of rat cerebellum using chicken anti-MAP2 antibody
Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Shootin-1 with Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/ 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.
-ve control 2: ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Shootin-1 with Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/ab5392 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.
-ve control 2: ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.
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