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AB319980

Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free

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Chicken Recombinant Monoclonal MAP2 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Mtap2, Map2, Microtubule-associated protein 2, MAP-2, Mtap2, Map2, Microtubule-associated protein 2, MAP-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)

ab319980 staining MAP2 in Rat hippocampal neurons (positive) and SV40LT-SMC (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab319980 at 1µg/ml and ab202272, Alexa Fluor 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)

ab319980 staining MAP2 in Mouse hippocampal neurons (positive) and NIH/3T3 (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab319980 at 1µg/ml and ab202272, Alexa Fluor 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)

Immunofluorescence staining of MAP2 in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab319980 at 1/100 dilution, and then incubated for 1 hour with ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free (AB319980)

Immunofluorescence staining of MAP2 in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab319980 at 1/100 dilution, and then incubated for 1 hour with ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Unconjugated

    Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • Carrier free

    Anti-MAP2 antibody [EPR19691] - BSA and Azide free

  • Unconjugated

    Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric)

  • Carrier free

    Anti-MAP2 antibody [EPR19691] - Mouse IgG2a (Chimeric) - BSA and Azide free

  • Unconjugated

    Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Goat IgG (Chimeric)

  • Carrier free

    Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

  • Unconjugated

    Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Chicken IgY (Chimeric)

  • Carrier free

    Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) - BSA and Azide free

  • Unconjugated

    Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Chicken IgY (Chimeric)

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

EPR19691

Isotype

IgY

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab319980 is the carrier free version of ab319979.

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab183830). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Thiophilic Resin
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
See full target information Map2

Additional targets

Map2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com