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Goat Recombinant Monoclonal MAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.


Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (AB302487), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (AB302487), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (AB302487), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (AB302487), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (AB302487), expandable thumbnail

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Recommended products

Goat Recombinant Monoclonal MAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19691

Purification technique

Ion exchange chromatography

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This goat monoclonal chimeric antibody has been engineered from RabMab parent antibody (Anti-MAP2 antibody [EPR19691] ab183830). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using across absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the rat colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the mouse colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the human colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a ready to use . Secondary antibody: Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723).Positive staining on mouse cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use .Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] – Goat IgG (Chimeric) (ab302487)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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