Goat Recombinant Monoclonal MAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Mouse | Tested | Tested |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Microtubule-associated protein 2, MAP-2, MAP2
Goat Recombinant Monoclonal MAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR19691
Ion exchange chromatography
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This goat monoclonal chimeric antibody has been engineered from RabMab parent antibody (Anti-MAP2 antibody [EPR19691] ab183830). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using across absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the rat colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the mouse colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the human colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a ready to use . Secondary antibody: Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723).Positive staining on mouse cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use .Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com