Name: Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric)
SKU: ab302487
Rating: 5 (1 reviews)

Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487) is a goat monoclonal antibody that is used to detect MAP2 in IHC-P, ICC/IF. Suitable for mouse, human, rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (AB302487), expandable thumbnail

Key facts

Isotype: IgG
Host species: Goat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF
Human	Tested	Expected
Mouse	Tested	Tested
Rat	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information MAP2

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Microtubule-associated protein 2, MAP-2, MAP2

Key facts

Isotype: IgG
Host species: Goat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR19691
Purification technique: Ion exchange chromatography
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?
Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487) is a goat recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in House Mouse, Human, Norway Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19691] also available for your convenience: ab183830, Alexa Fluor® 647 - ab225315, Alexa Fluor® 488 - ab225316, Carrier free - ab236033, ab300645, Carrier free - ab300646, ab302487, Carrier free - ab302488, Alexa Fluor® 568 - ab303465

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the human colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a ready to use . Secondary antibody: Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723).Positive staining on mouse cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use .Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the mouse colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on ganglion in the rat colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) (ab302487)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling MAP2 with ab302487 at 1/100 (10.04 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.

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Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric)

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