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Rabbit Recombinant Monoclonal MAP2 antibody. Suitable for IP, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] (AB221693), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MAP2 antibody [EPR22036-127] (AB221693), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (AB221693), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (AB221693), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (AB221693), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Expected
Expected
Predicted
Mouse
Expected
Tested
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Microtubule-associated protein 2, MAP-2, MAP2

Recommended products

Rabbit Recombinant Monoclonal MAP2 antibody. Suitable for IP, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22036-127

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    MAP2 was immunoprecipitated from 0.35 mg SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate 10 ug with ab221693 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab221693 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab221693 IP in SK-N-BE(2) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221693 in SK-N-BE(2) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds

    Fresh made lysate is required to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Predicted band size: 199 kDa

  • Flow Cytometry (Intracellular) - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse brain cells cells labelling MAP2 with ab221693 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling MAP2 with ab221693 at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control: Hepa1-6 cells (PMID: 7001466)

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling MAP2 (Green) using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Cytoplasmic staining on mouse cerebrum (PMID: 22479190) is observed. The nuclear counterstain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse kidney tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse kidney (PMID: 7001466, PMID: 2423532) is observed. The nuclear counterstain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

    Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse liver tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse liver (PMID: 7001466, PMID: 2423532). is observed. The nuclear counterstain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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