Skip to main content

Rabbit Recombinant Monoclonal MAP2 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22641-16] (AB254264), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (AB254264), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (AB254264), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (AB254264), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (AB254264), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Predicted
Expected
Tested
Mouse
Not recommended
Not recommended
Not recommended
Expected
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Predicted
Tested
Tested
Common marmoset
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Predicted

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Common marmoset
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Common marmoset
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Common marmoset
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Rat, Human, Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
1/500
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Common marmoset
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Microtubule-associated protein 2, MAP-2, MAP2

Recommended products

Rabbit Recombinant Monoclonal MAP2 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22641-16
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Find all reagents to label mature neurons in our "Mature neuron markers guide".

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab254264 at 1/2000 (0.299 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining on rat cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining on rat cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in neuron of human colon (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in rat cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com