Anti-MAP2 antibody [HM-2]

40 product images

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  Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

  Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

  Rivastigmine preserves neuronal morphology and alters sAPP secretion
  

  At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

  MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

  (From Figure 2A of Bailey et al)

  This image was generated using the ascites version of the product.

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  Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.
  

  This image was generated using the ascites version of the product.

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  Western blot - Anti-MAP2 antibody [HM-2] (ab11267)

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/mL

  All lanes: Rat brain lysate

  Predicted band size: 199 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with Anti-MAP2 antibody [RM1037] ab288714 at 1/500 (0.942 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling ADAM23 with Anti-ADAM23 antibody [EPR29232-65] ab322263 at 1/50 (10.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/ab11267 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-ADAM23 antibody [EPR29232-65] ab322263 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence

  Immunocytochemistry/Immunofluorescence analysis of mouse neuron cells labelling ABCA4 (Green) with Anti-ABCA4 antibody [EPR26543-66] ab303504 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor^® 488 conguated Goat Anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed, was used as the secondary antibody for the primary at a 1/1000 dilution. Anti-MAP2 mouse monoclonal antibody (ab11267) (Red) was used to counterstain at a 1/500 dilution, with Alexa Fluor^® 594 conguated Goat Anti-Mouse IgG H&L (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) as the secondary (1/1000 dilution). DAPI (Blue) was used as the nuclear counterstain.

  For negative (-ve) control 1, Anti-ABCA4 antibody [EPR26543-66] ab303504 (1/50 dilution) was used with secondary Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (1/1000 dilution) and -ve control 2 used ab11267 (1/500 dilution) with secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/1000 dilution).

  Confocal image showing no staining in mouse primary neuron.

  Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence

  Immunocytochemistry/Immunofluorescence analysis of rat retina cells labelling ABCA4 (Green) with Anti-ABCA4 antibody [EPR26543-66] ab303504 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor^® 488 conguated Goat Anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed, was used as the secondary antibody for the primary at a 1/1000 dilution. Anti-MAP2 mouse monoclonal antibody (ab11267) (Red) was used to counterstain at a 1/500 dilution, with Alexa Fluor^® 594 conguated Goat Anti-Mouse IgG H&L (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) as the secondary (1/1000 dilution). DAPI (Blue) was used as the nuclear counterstain.

  For negative (-ve) control 1, Anti-ABCA4 antibody [EPR26543-66] ab303504 (1/50 dilution) was used with secondary Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (1/1000 dilution) and -ve control 2 used ab11267 (1/500 dilution) with secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/1000 dilution).

  Confocal image showing cytoplasmic staining in rat retina primary neuron.

  Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labelling Parvalbumin with Anti-Parvalbumin antibody [EPR23455-66] - BSA and Azide free (Detector) ab281008 at 1/500 (2.074 μg/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in a subset of mouse primary neuron.

  Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 (4μg/ml) dilution, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used as the counterstain secondary antibody at 1/1000 (2μg/ml) (Magenta).

  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling SNAP25 with Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 (1.022 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  
  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling SNAP25 with Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 (1.022 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  
  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in part of the mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  
  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse hippocampal neuron cells labelling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in mouse hippocampus neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  -ve control 1: Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with Anti-TBR2 / Eomes antibody [RM2055] ab319166 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing nuclear staining in subsets of mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-TBR2 / Eomes antibody [RM2055] ab319166 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.

  -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling BRN3A + BRN3B + BRN3C with Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 AlexaFluor®488 Goat anti-Rabbit secondary preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing nuclear staining in rat primary neurons (shown in green). Counterstained with ab11267 anti-MAP2 (mouse mAb) at 1/1000 dilution (1 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution (2 μg/ml) (Magenta). Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  -ve control 1: Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at a 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution (2 μg/ml).
  -ve control 2: ab11267 at a 1/500 dilution (4 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution (2 μg/ml).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling BRN3A + BRN3B + BRN3C with Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 AlexaFluor®488 Goat anti-Rabbit secondary preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing nuclear staining in mouse primary neurons (shown in green). Counterstained with ab11267 anti-MAP2 (mouse mAb) at 1/1000 dilution (1 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution (2 μg/ml) (Magenta). Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  -ve control 1: Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at a 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution (2 μg/ml).
  -ve control 2: ab11267 at a 1/500 dilution (4 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution (2 μg/ml).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/100 (9.72 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse primary neuron cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/100 (9.72 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Piccolo with Anti-Piccolo antibody [EPR26840-80] ab307656 at 1/50 (10.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing synaptic staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Piccolo with Anti-Piccolo antibody [EPR26840-80] ab307656 at 1/50 (10.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing synaptic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labelling
  Parvalbumin with Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) ab281158 at 1/500 (1.992 μg/ml) dilution (Green).
  Confocal image showing cytoplasmic and nuclear staining in a subset of mouse primary neuron.
  
  

  Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 (4μg/ml) dilution, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used as the counterstain secondary antibody at 1/1000 (2μg/ml) (Magenta).
  
  

  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Tau (MBD region) with Anti-Tau (MBD region) antibody [EPR25205-233] ab308439 at 1/50 (9.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Tau (MBD region) with Anti-Tau (MBD region) antibody [EPR25205-233] ab308439 at 1/50 (9.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Synaptogyrin 1 with Anti-Synaptogyrin 1 antibody [EPR25081-18] ab308617 at 1/100 (4.985 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary neural/glia cell. Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Synaptogyrin 1 with Anti-Synaptogyrin 1 antibody [EPR25081-18] ab308617 at 1/100 (4.985 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilised Mouse primary retina with Anti-RS1 antibody [EPR28513-78] ab314231 (green) at 1/50 dilution (10.52 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). ab11267, Anti-MAP2 mouse monoclonal antibody was used as MAP2 counterstain (red) at 1/500 dilution (4 µg/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) secondary antibody used at 1/1000 dilution (2 µg/ml). Nuclei were counterstained with DAPI (blue).
  Confocal image showing cytoplasmic staining in cultured mouse primary retina cells.
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  Image was taken with a confocal microscope (Leica - Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilised Rat primary retina with Anti-RS1 antibody [EPR28513-78] ab314231 (green) at 1/50 dilution (10.52 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). ab11267, Anti-MAP2 mouse monoclonal antibody was used as MAP2 counterstain (red) at 1/500 dilution (4 µg/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) secondary antibody used at 1/1000 dilution (2 µg/ml). Nuclei were counterstained with DAPI (blue).
  Confocal image showing cytoplasmic staining in cultured rat primary retina cells.
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  Image was taken with a confocal microscope (Leica - Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)
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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)
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  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody [HM-2] (ab11267)
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [HM-2] (ab11267)
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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Alexa Fluor® 488 Anti-Calbindin antibody [EP3478] ab208377 staining Mouse hippocampal primary neural/glia cell. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and then counterstained using ab11267 Anti-MAP2 antibody [HM-2] at a 1/200 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor ^® 594) preabsorbed at a 1/1000 dilution. The cells were then incubated with Alexa Fluor® 488 Anti-Calbindin antibody [EP3478] ab208377 at 1/50 dilution (shown in green) Nuclear DNA was labelled with DAPI (shown in blue).

  Confocal image showing positive staining in subsets of mouse hippocampal primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis was performed on 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse hippocampal primary neural/glia cells labelling Calbindin with Anti-Calbindin antibody [EP3478] ab108404 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at dilution of 1/1000 (green).

  Anti-MAP2 antibody [HM-2] (ab11267) at 1/1000 dilution was used as a counterstain, with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as the secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at dilution of 1/1000 (red).

  Negative control 1: Anti-Calbindin (Anti-Calbindin antibody [EP3478] ab108404) at 1/250 dilution with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed as the secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at dilution of 1/1000.

  Negative control 2: Anti-MAP2 antibody [HM-2] (ab11267) at 1/200 dilution with Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at dilution of 1/1000.

  The nuclear counterstain is DAPI (blue).

  Confocal image showing positive staining in subsets of mouse hippocampal primary neuron.

  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Pan Trk with Anti-Pan Trk antibody [RM1032] ab314906 at 1/200 (2.445 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Pan Trk with Anti-Pan Trk antibody [RM1032] ab314906 at 1/200 (2.445 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
  Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling NeuroD1 with primary antibody anti-NeuroD1 (Anti-NeuroD1 antibody [EPR17084] ab205300) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing nuclear staining in part of mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 mouse monoclonal antibody (ab11267) was used to counterstain at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) as a secondary counterstain antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).
  The negative controls are as follows:-
  Negative control 1: Anti-NeuroD1 antibody [EPR17084] ab205300 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
  Negative control 2: ab11267 Anti-MAP2 mouse monoclonal antibody at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling GAD65 + GAD67 with Alexa Fluor® 488 Anti-GAD65 + GAD67 antibody [EPR19366] ab314811 at 1/50 (10.0 ug/ml) dilution.
  Confocal image showing cytoplasmic staining in rat primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labeling pan PDE4 with Anti-pan PDE4 antibody [EPR25202-101] ab300108 at 1/50 (10.44 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4µg/ml) dilution (red) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. The Nuclear counterstain was DAPI (blue).
  -ve control: ab11267 at 1/500 (4μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 (2 ug/ml) dilution.