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Anti-MAP2 antibody [HM-2] (ab11267) is a mouse monoclonal antibody detecting MAP2 in Western Blot. Suitable for Rat.



- Over 170 publications

- Trusted since 2004



Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (AB11267), expandable thumbnail
  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (AB11267), expandable thumbnail
  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (AB11267), expandable thumbnail
  • Western blot - Anti-MAP2 antibody [HM-2] - Neuronal Marker (AB11267), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (AB11267), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Rat Map2. The exact immunogen used to generate this antibody is proprietary information. Database link P15146

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Tested
Tested
Chicken
Predicted
Predicted
Cow
Predicted
Predicted
Quail
Predicted
Predicted

Tested
Tested

Species
Rat
Dilution info
2 µg/mL
Notes

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .

Predicted
Predicted

Species
Mouse, Chicken, Cow, Human, Quail
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Chicken, Cow, Human, Quail
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Recommended products

Anti-MAP2 antibody [HM-2] (ab11267) is a mouse monoclonal antibody detecting MAP2 in Western Blot. Suitable for Rat.



- Over 170 publications

- Trusted since 2004


Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Rat Map2. The exact immunogen used to generate this antibody is proprietary information. Database link P15146
Clone number
HM-2
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-MAP2 antibody [HM-2] (ab11267) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Rat samples.

What is the molecular weight of MAP2?


Anti-MAP2 [HM-2] (ab11267) specifically detects a band for MAP2 (UniProt: P11137) at a molecular weight of 200kDa.

Trusted by the scientific community


Anti-MAP2 [HM-2] (ab11267) was first used in a scientific publication in 2004 and has been cited over 170 times in peer-reviewed journals.

Reviewed by scientists


Anti-MAP2 [HM-2] (ab11267) has over 5 independent reviews from customers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

41 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Neogenin with Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing positive staining in mouse primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail
    Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry staining using mouse Anti-MAP2 antibody

    Rivastigmine preserves neuronal morphology and alters sAPP secretion

    At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

    MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

    (From Figure 2A of Bailey et al)

    This image was generated using the ascites version of the product.

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry staining using mouse Anti-MAP2 antibody

    Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Western blot - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Western blot staining of Rat brain lysate using mouse Anti-MAP2 antibody

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267) at 1 µg/mL

    All lanes: Rat brain lysate

    Predicted band size: 199 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron cells using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with Anti-MAP2 antibody [RM1037] - Neuronal Marker ab288714 at 1/500 (0.942 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cells using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution (Magenta).

    -ve control 1: Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cells using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution (Magenta).

    -ve control 1: Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunohistochemistry (PFA perfusion fixed frozen sections) staining using mouse Anti-MAP2 antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-MAP2 antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat neuron cells using mouse Anti-MAP2 antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat retina cells using mouse Anti-MAP2 antibody

    Immunocytochemistry/Immunofluorescence analysis of rat retina cells labelling ABCA4 (Green) with Anti-ABCA4 antibody [EPR26543-66] ab303504 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor® 488 conguated Goat Anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed, was used as the secondary antibody for the primary at a 1/1000 dilution. Anti-MAP2 mouse monoclonal antibody (ab11267) (Red) was used to counterstain at a 1/500 dilution, with Alexa Fluor® 594 conguated Goat Anti-Mouse IgG H&L (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) as the secondary (1/1000 dilution). DAPI (Blue) was used as the nuclear counterstain.

    For negative (-ve) control 1, Anti-ABCA4 antibody [EPR26543-66] ab303504 (1/50 dilution) was used with secondary Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (1/1000 dilution) and -ve control 2 used ab11267 (1/500 dilution) with secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/1000 dilution).

    Confocal image showing cytoplasmic staining in rat retina primary neuron.

    Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse neuron cells using mouse Anti-MAP2 antibody

    Immunocytochemistry/Immunofluorescence analysis of mouse neuron cells labelling ABCA4 (Green) with Anti-ABCA4 antibody [EPR26543-66] ab303504 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor® 488 conguated Goat Anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed, was used as the secondary antibody for the primary at a 1/1000 dilution. Anti-MAP2 mouse monoclonal antibody (ab11267) (Red) was used to counterstain at a 1/500 dilution, with Alexa Fluor® 594 conguated Goat Anti-Mouse IgG H&L (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) as the secondary (1/1000 dilution). DAPI (Blue) was used as the nuclear counterstain.

    For negative (-ve) control 1, Anti-ABCA4 antibody [EPR26543-66] ab303504 (1/50 dilution) was used with secondary Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (1/1000 dilution) and -ve control 2 used ab11267 (1/500 dilution) with secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/1000 dilution).

    Confocal image showing no staining in mouse primary neuron.

    Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse retina cells using mouse Anti-MAP2 antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labelling
    Parvalbumin with Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) ab281158 at 1/500 (1.992 μg/ml) dilution (Green).
    Confocal image showing cytoplasmic and nuclear staining in a subset of mouse primary neuron.

    Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 (4μg/ml) dilution, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used as the counterstain secondary antibody at 1/1000 (2μg/ml) (Magenta).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labelling Parvalbumin with Anti-Parvalbumin antibody [EPR23455-66] - BSA and Azide free (Detector) ab281008 at 1/500 (2.074 μg/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in a subset of mouse primary neuron.

    Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 (4μg/ml) dilution, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used as the counterstain secondary antibody at 1/1000 (2μg/ml) (Magenta).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse primary neuron cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/100 (9.72 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/100 (9.72 ug/ml) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with Anti-TBR2 / Eomes antibody [RM2055] ab319166 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing nuclear staining in subsets of mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-TBR2 / Eomes antibody [RM2055] ab319166 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cells using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling BRN3A + BRN3B + BRN3C with Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 AlexaFluor®488 Goat anti-Rabbit secondary preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing nuclear staining in mouse primary neurons (shown in green). Counterstained with ab11267 anti-MAP2 (mouse mAb) at 1/1000 dilution (1 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution (2 μg/ml) (Magenta). Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    -ve control 1: Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at a 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution (2 μg/ml).
    -ve control 2: ab11267 at a 1/500 dilution (4 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution (2 μg/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cells using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling BRN3A + BRN3B + BRN3C with Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 AlexaFluor®488 Goat anti-Rabbit secondary preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing nuclear staining in rat primary neurons (shown in green). Counterstained with ab11267 anti-MAP2 (mouse mAb) at 1/1000 dilution (1 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution (2 μg/ml) (Magenta). Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    -ve control 1: Anti-BRN3A + BRN3B + BRN3C antibody [EPR26313-54] ab317492 at a 1/200 dilution (2.575 μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution (2 μg/ml).
    -ve control 2: ab11267 at a 1/500 dilution (4 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution (2 μg/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse hippocampus neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse hippocampal neuron cells labelling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in mouse hippocampus neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    -ve control 1: Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neurons using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in part of the mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling SNAP25 with Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 (1.022 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling SNAP25 with Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 (1.022 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-SNAP25 antibody [RM1207] ab320654 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling ADAM23 with ab322263 at 1/50 (10.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/ab11267 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab322263 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized rat primary neuron cells labelling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling TrkB with Anti-TrkB antibody [RM1253] ab322464 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-TrkB antibody [RM1253] ab322464 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody preadsorbed at 1/1000 2 ug/ml dilution
    -ve control 2: ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution , followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling TrkB with Anti-TrkB antibody [RM1253] ab322464 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-TrkB antibody [RM1253] ab322464 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody preadsorbed at 1/1000 2 ug/ml dilution
    -ve control 2: ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution , followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26422-118] ab314307 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial rat primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labeling pan PDE4 with Anti-pan PDE4 antibody [EPR25202-101] ab300108 at 1/50 (10.44 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4µg/ml) dilution (red) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. The Nuclear counterstain was DAPI (blue).
    -ve control: ab11267 at 1/500 (4μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron labeling pan PDE4 with Anti-pan PDE4 antibody [EPR25202-101] ab300108 at 1/50 (10.44 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4µg/ml) dilution (red) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. The Nuclear counterstain was DAPI (blue).
    -ve control: ab11267 at 1/500 (4μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling nNOS (neuronal) with Anti-nNOS (neuronal) antibody [RM1237] ab323183 at 1/2000 (0.253 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2ug/ml) dilution (Magenta).

    -ve control 1: Anti-nNOS (neuronal) antibody [RM1237] ab323183 at a 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.

    -ve control 2: ab11267 at a 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of Rat primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling GAD65 + GAD67 with Alexa Fluor® 488 Anti-GAD65 + GAD67 antibody [EPR19366] ab314811 at 1/50 (10.0 ug/ml) dilution.
    Confocal image showing cytoplasmic staining in rat primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling nNOS (neuronal) with Anti-nNOS (neuronal) antibody [RM1237] ab323183 at 1/2000 (0.253 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neural/glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2ug/ml) dilution (Magenta).

    -ve control 1: Anti-nNOS (neuronal) antibody [RM1237] ab323183 at a 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.

    -ve control 2: ab11267 at a 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
    Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Pan Trk with Anti-Pan Trk antibody [RM1032] ab314906 at 1/200 (2.445 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] - Neuronal Marker (ab11267)

    MAP2 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-MAP2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Pan Trk with Anti-Pan Trk antibody [RM1032] ab314906 at 1/200 (2.445 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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