Anti-MAP2 antibody ab32454 is a rabbit polyclonal antibody that is used in MAP2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Tried and trusted by researchers since 2007
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- Nature communications 14:769620233D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonbin Park,Jae-Seong Lee,Ge Gao,Byoung Soo Kim,Dong-Woo ChoPubMed 38001146
- Nature communications 14:53412023Ascl1 and Ngn2 convert mouse embryonic stem cells to neurons via functionally distinct paths.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGintautas Vainorius,Maria Novatchkova,Georg Michlits,Juliane Christina Baar,Cecilia Raupach,Joonsun Lee,Ramesh Yelagandula,Marius Wernig,Ulrich EllingPubMed 37660160
- Alzheimer's & dementia : the journal of the Alzheimer's Association 19:4407-44202023CALHM2 V136G polymorphism reduces astrocytic ATP release and is associated with depressive symptoms and Alzheimer's disease risk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liao,Yingyi Wang,Qing-Qing Tao,Chaoguang Yang,Jinlei Wang,Jinbo Cheng,Jun Ma,Zhi-Ying Wu,Rui-Yuan Pan,Zengqiang YuanPubMed 37493186
- Nature metabolism 5:1290-13022023Fatty acid oxidation organizes mitochondrial supercomplexes to sustain astrocytic ROS and cognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrenda Morant-Ferrando,Daniel Jimenez-Blasco,Paula Alonso-Batan,Jesús Agulla,Rebeca Lapresa,Dario Garcia-Rodriguez,Sara Yunta-Sanchez,Irene Lopez-Fabuel,Emilio Fernandez,Peter Carmeliet,Angeles Almeida,Marina Garcia-Macia,Juan P BolañosPubMed 37460843
- Bioactive materials 29:196-2132023FUS-mediated HypEVs: Neuroprotective effects against ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYousheng Wu,Xiaoxiong Huang,Zefeng Tan,Jiankun Zang,Min Peng,Niu He,Tao Zhang,Hongcheng Mai,Anding Xu,Dan LuPubMed 37621770
- Cellular and molecular neurobiology 43:3575-35922023BMECs Ameliorate High Glucose-Induced Morphological Aberrations and Synaptic Dysfunction via VEGF-Mediated Modulation of Glucose Uptake in Cortical Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Qi Huang,Xiao Gu,Xiao Chen,Yi-Ting Du,Bin-Chi Chen,Feng-Yan SunPubMed 37418138
- Neuroscience bulletin 39:1638-16542023Genetic Architecture and Functional Implications of the CSF-Contacting Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyuan Song,Yumin Yuan,Lingling Xu,Jun Jiang,Ying Li,Yao Yan,Qing Li,Fang Zhou,Junli Cao,Licai ZhangPubMed 37405574
- Journal of clinical biochemistry and nutrition 73:191-1972023Induction of -amplified neuroblastoma differentiation through NMYC suppression using PPAR-γ antagonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukako Nakao-Ise,Takumi Narita,Shingo Miyamoto,Motoki Watanabe,Takuji Tanaka,Yoshihiro Sowa,Yosuke Iizumi,Mitsuharu Masuda,Gen Fujii,Yasuko Hirai,Toshimasa Nakao,Hideki Takakura,Michihiro MutohPubMed 37970556
- Pharmaceuticals (Basel, Switzerland) 16:2023Chlorogenic Acid Induced Neuroblastoma Cells Differentiation via the ACAT1-TPK1-PDH Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShen You,Ming-Jin Wang,Zhen-Yan Hou,Wei-Da Wang,Ting-Ting Du,Ni-Na Xue,Ming Ji,Xiao-Guang ChenPubMed 37375824
- iScience 26:1065342023General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnqiang Chang,Yangyang Wang,Ruilou Zhu,Lingzhi Wu,Yitian Yang,Shuang Zeng,Ningtao Li,Xiaoguo Ruan,Mingyang Sun,Wei Zhang,Jun Zhou,Mengrong Miao,Hui Zhi,Hailin Zhao,Qian Chen,Qizhe Sun,Emer Chang,Albert Chang,Tingting Zhang,Xinfang He,Kan Liu,Songhua Ma,Weizhong Zhu,Youming Zhang,Luca Magnani,Daqing Ma,Jiaqiang ZhangPubMed 37123239
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Predicted | Expected | Expected |
| Mouse | Tested | Expected | Tested |
| Rat | Expected | Tested | Tested |
| Cat | Predicted | Predicted | Predicted |
| Goat | Predicted | Predicted | Predicted |
| Lizard | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat, Cat, Human, Lizard | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat, Cat, Lizard | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat, Cat, Lizard | Dilution info - | Notes - |
Associated Products
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Target data
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Alternative names
Microtubule-associated protein 2, MAP-2, MAP2
Recommended products
Anti-MAP2 antibody ab32454 is a rabbit polyclonal antibody that is used in MAP2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Tried and trusted by researchers since 2007
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Müller A et al. Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling. PLoS One 10:e0145451 (2015).Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (ab32454)
Rat astrocytes (DIV 22) cells were fixed with 4% paraformaldehyde and stained for MAP2 (Green) using ab32454 at 1/1000 dilution in ICC/IF analysis.
Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454)
Blocking buffer: 2% BSA
Gel type: TA
All lanes: Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454) at 1 µg/mL
Lane 1: Mouse Brain (day 0) Tissue Lysate at 20 µg
Lane 2: Mouse Brain Tissue Lysate at 20 µg
Lane 3: Rat Brain Tissue Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 199 kDa
Observed band size: 268 kDa, 280 kDa
Exposure time: 2min
This image is courtesy of a customer review by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody - Neuronal Marker (ab32454)
ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Gonzales PK et al. Transcriptome analysis of genetically matched human induced pluripotent stem cells disomic or trisomic for chromosome 21. PLoS One 13:e0194581 (2018).Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454)
All lanes: Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454) at 1/1000 dilution
Lanes 1 - 2: Trisomic induced Pluripotent Stem Cells (iPSCs)
Lanes 3 - 4: Disomic induced Pluripotent Stem Cells (iPSCs)
Predicted band size: 199 kDa
This image is courtesy of a customer review submitted by Babben TinnerImmunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (ab32454)
ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody - Neuronal Marker (ab32454)
IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (ab32454)
ab32454 staining MAP2 in Rat Primary Neurons DIV14 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32454 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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