Anti-MAP2 antibody - Neuronal Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB5392)

Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

• ICC

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Immunocytochemistry - Anti-MAP2 antibody - Neuronal Marker (AB5392)

ab5392 at a dilution of 1/10000 staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with DAPI.

• ICC/IF

AbReview16187****

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB5392)

ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor^® 488 goat polyclonal to chicken IgY diluted 1/400 was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• ICC

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Immunocytochemistry - Anti-MAP2 antibody - Neuronal Marker (AB5392)

Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1 : 30 000) a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB5392)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Shootin-1 with ab322654 at 1/100 (5.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/ 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322654 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution. -ve control 2 : ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB5392)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Shootin-1 with ab322654 at 1/100 (5.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322654 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution. -ve control 2 : ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody - Neuronal Marker (AB5392)

Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.

• WB

AbReview33335****

Western blot - Anti-MAP2 antibody - Neuronal Marker (AB5392)

All lanes:

Western blot - Anti-MAP2 antibody - Neuronal Marker (ab5392) at 1/10000 dilution

All lanes:

Mouse brain lysate - post nuclear supernatant at 10 µg

Secondary

All lanes:

HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution

Predicted band size: 199 kDa

Observed band size: 280 kDa,70 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-MAP2 antibody - Neuronal Marker (AB5392)

All lanes:

Western blot - Anti-MAP2 antibody - Neuronal Marker (ab5392) at 1/50000 dilution

Lane 1:

Adult rat brain lysate

Lane 2:

Embryonic E20 rat brain lysate

Lane 3:

Adult mouse brain lysate

Predicted band size: 199 kDa

false