Anti-MAP2 antibody (ab92434) is a chicken polyclonal antibody that is used to detect MAP2 in Western Blot. Suitable for Human, Rat samples.
- Over 60 publications
- Trusted since 2010
Preservative: 0.065% Sodium azide
ICC | WB | |
---|---|---|
Human | Tested | Expected |
Rat | Expected | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/20000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Microtubule-associated protein 2, MAP-2, MAP2
Anti-MAP2 antibody (ab92434) is a chicken polyclonal antibody that is used to detect MAP2 in Western Blot. Suitable for Human, Rat samples.
- Over 60 publications
- Trusted since 2010
Preservative: 0.065% Sodium azide
Find all reagents to label mature neurons in our "Mature neuron markers guide".
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.
MAP2 Immunocytochemistry staining using chicken Anti-MAP2 antibody
ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
MAP2 Western blot staining of Rat cerebral cortex lysate using chicken Anti-MAP2 antibody
All lanes: Western blot - Anti-MAP2 antibody - Neuronal Marker (ab92434) at 1/20000 dilution
All lanes: Rat cerebral cortex lysate
Predicted band size: 199 kDa
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