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Rabbit Recombinant Multiclonal MAP2 antibody. Neuron marker. Suitable for ICC, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Immunocytochemistry - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588), expandable thumbnail
  • Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Expected
Not recommended
Tested
Not recommended
Expected
Expected
Mouse
Tested
Not recommended
Tested
Not recommended
Tested
Tested
Rat
Expected
Not recommended
Tested
Not recommended
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Rat

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Recommended products

Rabbit Recombinant Multiclonal MAP2 antibody. Neuron marker. Suitable for ICC, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1010

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Immunocytochemistry - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with 281588 at 1/2000 (0.276 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Mouse primary neuron cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution

    Lane 1: SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 199 kDa

    Observed band size: 280 kDa, 70 kDa

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

    Exposure time: 3 seconds.

    All lanes: Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution

    Lane 1: Mouse E12.5 brain lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Lane 3: Mouse cerebellum lysate at 20 µg

    Lane 4: Rat brain lysate at 20 µg

    Lane 5: Rat cerebellum lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 199 kDa

    Observed band size: 280 kDa, 70 kDa

  • Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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