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AB32134

Anti-MAP2K1IP1 antibody [Y130]

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(5 Publications)

Rabbit Recombinant Monoclonal MAP2K1IP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

MAP2K1IP1, MAPKSP1, PRO2783, LAMTOR3, Ragulator complex protein LAMTOR3, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, MEK-binding partner 1, Mitogen-activated protein kinase kinase 1-interacting protein 1, Mitogen-activated protein kinase scaffold protein 1, Mp1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (AB32134)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (AB32134)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling MAP2K1IP1 with purified ab32134 at 1/100 dilution (10 μg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134)

ab32134 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134)

ab32134, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134)
  • WB

Lab

Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134)

Lanes 1-2 : Merged signal (red and green). Green - ab32134 observed at 14 kDa. Red - loading control ab8245 observed at 37 kDa.

ab32134 Anti-MAP2K1IP1 antibody [Y130] was shown to specifically react with MAP2K1IP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265624 (knockout cell lysate ab258021) was used. Wild-type and MAP2K1IP1 knockout samples were subjected to SDS-PAGE. ab32134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 50000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MAP2K1IP1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lamtor3-map2k1ip1-knockout-hela-cell-line-ab265624'>ab265624</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134)
  • WB

Unknown

Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134)

All lanes:

Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution

All lanes:

A431 cell lysate

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y130

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32134 recognises MP1 (MEK partner-1).

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Complementary Products

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [EPR16667] (AB178876)

  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB32517

Anti-MEK2 antibody [Y78]

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Flow Cytometry (Intracellular) - Anti-MEK2 antibody [Y78] (AB32517)

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Primary Antibodies

AB200653

Anti-A-Raf antibody [EPR16208]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A-Raf antibody [EPR16208] (AB200653)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed : 20381137, PubMed : 22980980, PubMed : 28935770, PubMed : 29107538, PubMed : 29123114, PubMed : 29158492, PubMed : 30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) : it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed : 22980980, PubMed : 28935770, PubMed : 29107538, PubMed : 29123114, PubMed : 29158492, PubMed : 30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed : 22980980, PubMed : 28935770, PubMed : 29107538, PubMed : 29123114, PubMed : 29158492, PubMed : 30181260). Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 (By similarity).
See full target information LAMTOR3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell research : PubMed32839552

2020

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shen'ao Zhou,Wei Zhang,Gaihong Cai,Yingzhe Ding,Caixia Wei,Sheng Li,Yu Yang,Jie Qin,Dan Liu,Hao Zhang,Xiexiang Shao,Jianhua Wang,Hongye Wang,Wenjun Yang,Huating Wang,She Chen,Ping Hu,Liming Sun

Medical science monitor : international medical jo 25:8905-8912 PubMed31760402

2019

Napabucasin Attenuates Resistance of Breast Cancer Cells to Tamoxifen by Reducing Stem Cell-Like Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Xueni Liu,Jianhui Huang,Yanru Xie,Yuefen Zhou,Renyi Wang,Jian Lou

Oncology letters 18:314-320 PubMed31289502

2019

miR-330-3p suppresses liver cancer cell migration by targeting MAP2K1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Jin,Baoxing Jia,Ludong Tan,Yahui Liu

The Journal of cell biology 205:525-40 PubMed24841562

2014

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Applications

IP

Species

Human

Natalia Schiefermeier,Julia M Scheffler,Mariana E G de Araujo,Taras Stasyk,Teodor Yordanov,Hannes L Ebner,Martin Offterdinger,Sebastian Munck,Michael W Hess,Sara A Wickström,Anika Lange,Winfried Wunderlich,Reinhard Fässler,David Teis,Lukas A Huber

The Journal of experimental medicine 208:2675-89 PubMed22143885

2011

A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Bart A Westerman,A Koen Braat,Nicole Taub,Marko Potman,Joseph H A Vissers,Marleen Blom,Els Verhoeven,Hans Stoop,Ad Gillis,Arno Velds,Wouter Nijkamp,Roderick Beijersbergen,Lukas A Huber,Leendert H J Looijenga,Maarten van Lohuizen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com