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Rabbit Recombinant Monoclonal MAP2K1IP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134), expandable thumbnail
  • Western blot - Anti-MAP2K1IP1 antibody [Y130] (AB32134), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (AB32134), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:20381137, PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29123114, PubMed:29158492, PubMed:30181260). Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 (By similarity).

Alternative names

MAP2K1IP1, MAPKSP1, PRO2783, LAMTOR3, Ragulator complex protein LAMTOR3, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, MEK-binding partner 1, Mitogen-activated protein kinase kinase 1-interacting protein 1, Mitogen-activated protein kinase scaffold protein 1, Mp1

Recommended products

Rabbit Recombinant Monoclonal MAP2K1IP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
Y130
Purification technique
Affinity purification Protein A
Specificity

ab32134 recognises MP1 (MEK partner-1).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134), expandable thumbnail

    Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    Lanes 1-2: Merged signal (red and green). Green - ab32134 observed at 14 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab32134 Anti-MAP2K1IP1 antibody [Y130] was shown to specifically react with MAP2K1IP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell line ab265624 (knockout cell lysate Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell lysate ab258021) was used. Wild-type and MAP2K1IP1 knockout samples were subjected to SDS-PAGE. ab32134 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 50000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: MAP2K1IP1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell line (Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell line ab265624)

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

  • Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134), expandable thumbnail

    Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    All lanes: Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution

    All lanes: A431 cell lysate

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (ab32134), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling MAP2K1IP1 with purified ab32134 at 1/100 dilution (10 μg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    ab32134, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    ab32134 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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