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Rabbit Recombinant Monoclonal MAP3K1 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-MAP3K1 antibody [EPR26099-13] (AB322196), expandable thumbnail
  • Western blot - Anti-MAP3K1 antibody [EPR26099-13] (AB322196), expandable thumbnail
  • Western blot - Anti-MAP3K1 antibody [EPR26099-13] (AB322196), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Component of a protein kinase signal transduction cascade (PubMed:9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:9808624).

Alternative names

MAPKKK1, MEKK, MEKK1, MAP3K1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1, MEK kinase 1, MEKK 1

Recommended products

Rabbit Recombinant Monoclonal MAP3K1 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26099-13
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196), expandable thumbnail

    Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting (Lane 3-4).

    The identity of the lower MW bands are likely to be degradation fragments.

    In Western blot, Anti-Vinculin antibody [EPR8185]- Loading Control (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/100000 dilution.

    Exposure time: Lane 1-2: 180 seconds; Lane 3-4: 59 seconds

    All lanes: Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell frozen lysate at 20 µg

    Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell frozen lysate at 20 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell fresh lysate at 20 µg

    Lane 4: EL4 (mouse lymphoma T lymphocyte) whole cell freshlysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 196 kDa, 124 kDa

  • Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196), expandable thumbnail

    Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting.

    In Western blot, Anti-Vinculin antibody [EPR8185]- Loading Control (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/100000 dilution.

    All lanes: Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196) at 1/1000 dilution

    Lane 1: Mouse brain fresh tissue lysate at 20 µg

    Lane 2: Mouse liver fresh tissue lysate at 20 µg

    Lane 3: Mouse testis fresh tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 196 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196), expandable thumbnail

    Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting.

    In Western blot, Anti-Vinculin antibody [EPR8185] - Loading Control (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/100000 dilution.

    All lanes: Western blot - Anti-MAP3K1 antibody [EPR26099-13] (ab322196) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell fresh lysate at 20 µg

    Lane 2: 293T transfected with siRNA specifically targeting MAP3K1 whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 196 kDa, 124 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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