Anti-MAP3K1 antibody [EPR26099-13] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-MAP3K1 antibody [EPR26099-13] - BSA and Azide free (AB324764)

This data was developed using ab322196, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting.

In Western blot Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-MAP3K1 antibody [EPR26099-13] (<a href='/en-us/products/primary-antibodies/map3k1-antibody-epr26099-13-ab322196'>ab322196</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell fresh lysate at 20 µg

Lane 2:

293T transfected with siRNA specifically targeting MAP3K1 whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 196 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MAP3K1 antibody [EPR26099-13] - BSA and Azide free (AB324764)

This data was developed using ab322196, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting (Lane 3-4).

The identity of the lower MW bands are likely to be degradation fragments.

In Western blot Anti-Vinculin antibody [EPR8185]- Loading Control (ab129002) staining at 1/100000 dilution.

Exposure time : Lane 1-2 : 180 seconds; Lane 3-4 : 59 seconds

All lanes:

Western blot - Anti-MAP3K1 antibody [EPR26099-13] (<a href='/en-us/products/primary-antibodies/map3k1-antibody-epr26099-13-ab322196'>ab322196</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell frozen lysate at 20 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell frozen lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell fresh lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma T lymphocyte) whole cell freshlysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 196 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-MAP3K1 antibody [EPR26099-13] - BSA and Azide free (AB324764)

This data was developed using ab322196, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells and tissues were lysed immediately after harvest and loaded onto membranes for Western blotting.

In Western blot Anti-Vinculin antibody [EPR8185]- Loading Control (ab129002) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-MAP3K1 antibody [EPR26099-13] (<a href='/en-us/products/primary-antibodies/map3k1-antibody-epr26099-13-ab322196'>ab322196</a>) at 1/1000 dilution

Lane 1:

Mouse brain fresh tissue lysate at 20 µg

Lane 2:

Mouse liver fresh tissue lysate at 20 µg

Lane 3:

Mouse testis fresh tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 196 kDa,124 kDa

false

Exposure time: 180s