AB138662

Anti-MAP3K1 (phospho T1400) antibody

Name: Anti-MAP3K1 (phospho T1400) antibody (ab138662) | Abcam
Brand: Abcam Limited
SKU: AB138662
Price: 545 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(8 Publications)

Rabbit Polyclonal MAP3K1 phospho T1400 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K1 phospho T1400.

View Alternative Names

MAPKKK1, MEKK, MEKK1, MAP3K1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1, MEK kinase 1, MEKK 1

2 Images

AbReview45489****

Western blot - Anti-MAP3K1 (phospho T1400) antibody (AB138662)

All lanes:

Western blot - Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/250 dilution

Lane 1:

Mouse B16F10 whole cell lysate - untreated at 60 µg

Lane 2:

Mouse B16F10 whole cell lysate - treated with 5µM Osteoponin for 10 minutes at 60 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 164 kDa

Observed band size: 150 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-MAP3K1 (phospho T1400) antibody (AB138662)

All lanes:

Western blot - Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/500 dilution

All lanes:

extracts from Jurkat cells at 30 µg

Predicted band size: 164 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MAP3K1 phospho T1400. The exact immunogen used to generate this antibody is proprietary information.

Q13233

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138662 was purified from Rabbit antiserum. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a protein kinase signal transduction cascade (PubMed : 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed : 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed : 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed : 9808624).

See full target information MAP3K1 phospho T1400

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:15 PubMed39901302

2025

Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Senhui Wei,Ying Li,Jing Zhou,Yongming Xia

Cell biology international 47:1198-1208 PubMed36950779

2023

RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating p38 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Deng,Jing Wu,Wei Zheng,Qian Wang,Dongling Li,Hongyu Kuang

Cell death discovery 7:240 PubMed34526481

2021

Histone deacetylase HDAC4 participates in the pathological process of myocardial ischemia-reperfusion injury via MEKK1/JNK pathway by binding to miR-206.

Applications

Unspecified application

Species

Unspecified reactive species

Qingman Li,Lijie Zhu,Fangqing Niu,Qingmin Li,Che Wang,Honghui Yang,Chuanyu Gao

Folia neuropathologica 58:45-56 PubMed32337957

2020

PARP1 might enhance the therapeutic effect of tetrahydroxystilbene glucoside in traumatic brain injury via inhibition of Ras/JNK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Cao,Yu Chen,Fei Wang,Yonggang Wang,Jiang Long

Artificial cells, nanomedicine, and biotechnology 47:3839-3846 PubMed31556312

2019

Sinomenine restrains breast cancer cells proliferation, migration and invasion via modulation of miR-29/PDCD-4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanglei Gao,Xiaolin Liang,Wenyan Ma

EMBO reports 19: PubMed30026307

2018

Planar cell polarity gene triggers apoptosis in neurodegenerative disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Li Li,Shaohong Peng,Francis M Chen,Qian Zhang,Ying An,Xiao Lin,Wen Li,Alex Chun Koon,Ting-Fung Chan,Kwok-Fai Lau,Jacky Chi Ki Ngo,Wing Tak Wong,Kin Ming Kwan,Ho Yin Edwin Chan

Journal of cellular biochemistry 119:6461-6469 PubMed29323740

2018

Long non-coding RNA ASBEL promotes osteosarcoma cell proliferation, migration, and invasion by regulating microRNA-21.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Zhao,Chao Zhang,Zhongli Gao,Han Wu,Rui Gu,Rui Jiang

The Journal of biological chemistry 289:11443-53 PubMed24599959

2014

p38δ regulates p53 to control p21Cip1 expression in human epidermal keratinocytes.

Applications

Species

Unspecified reactive species

Kamalika Saha,Gautam Adhikary,Santosh R Kanade,Ellen A Rorke,Richard L Eckert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP3K1 (phospho T1400) antibody

Western blot - Anti-MAP3K1 (phospho T1400) antibody (AB138662)

Western blot - Anti-MAP3K1 (phospho T1400) antibody (AB138662)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

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