Rabbit Polyclonal MAP3K4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K4 aa 50-150.
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Publications
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- Genes and environment : the official journal of the Japanese Environmental Mutagen Society 44:232022MicroRNA miR-627-5p restrains pulmonary artery smooth muscle cell dysfunction by targeting MAP 2 K4 and PI3K/AKT signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Li,Xiaoqin Tan,Yuexia Huang,Jun Cui,Fan Chen,Ying XiongPubMed 36163195
- Molecular medicine reports 22:1195-12042020Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxin Jia et. al.PubMed 32468015
- Oncology letters 18:2605-26112019Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Chen et. al.PubMed 31404324
- Oncology letters 17:2923-29302019Long non-coding RNA-OIS1 inhibits HPV-positive, but not HPV-negative cervical squamous cell carcinoma by upregulating MTK-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Zhou et. al.PubMed 30854069
- Oncology letters 16:3453-34582018Downregulation of ERBB3 decreases the proliferation, migration and invasion of cervical cancer cells though the interaction with MTK-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyun Du et. al.PubMed 30127948
- Nature structural & molecular biology 22:759-662015Histone monoubiquitination by Clock-Bmal1 complex marks Per1 and Per2 genes for circadian feedback.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAP3K4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R4
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Alternative names
KIAA0213, MAPKKK4, MEKK4, MTK1, MAP3K4, Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4
Recommended products
Rabbit Polyclonal MAP3K4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K4 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAP3K4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6R4
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab186125 was affinity purified using an epitope specific to MAP3K4 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-MAP3K4 antibody (ab186125)
All lanes: Western blot - Anti-MAP3K4 antibody (ab186125) at 0.1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 182 kDa
Exposure time: 3min
Immunoprecipitation - Anti-MAP3K4 antibody (ab186125)
Detection of MAP3K4 in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186125 at 6 μg/reaction for IP (Lane 1). For WB detection, ab186125 was used at 1μg/ml. Lane 2: Control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-MAP3K4 antibody (ab186125)
Predicted band size: 182 kDa
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