AB186125

Anti-MAP3K4 antibody

Name: Anti-MAP3K4 antibody (ab186125) | Abcam
Brand: Abcam Limited
SKU: AB186125
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal MAP3K4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K4 aa 50-150.

View Alternative Names

KIAA0213, MAPKKK4, MEKK4, MTK1, MAP3K4, Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4

2 Images

Supplier Data

Immunoprecipitation - Anti-MAP3K4 antibody (AB186125)

Detection of MAP3K4 in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186125 at 6 μg/reaction for IP (Lane 1). For WB detection, ab186125 was used at 1μg/ml. Lane 2 : Control IgG IP.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-MAP3K4 antibody (ab186125)

Predicted band size: 182 kDa

false

Supplier Data

Western blot - Anti-MAP3K4 antibody (AB186125)

All lanes:

Western blot - Anti-MAP3K4 antibody (ab186125) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 50 µg

Predicted band size: 182 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MAP3K4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6R4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab186125 was affinity purified using an epitope specific to MAP3K4 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

See full target information MAP3K4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:15 PubMed39901302

2025

Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Senhui Wei,Ying Li,Jing Zhou,Yongming Xia

Genes and environment : the official journal of the Japanese Environmental Mutagen Society 44:23 PubMed36163195

2022

MicroRNA miR-627-5p restrains pulmonary artery smooth muscle cell dysfunction by targeting MAP 2 K4 and PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Xiaoqin Tan,Yuexia Huang,Jun Cui,Fan Chen,Ying Xiong

Molecular medicine reports 22:1195-1204 PubMed32468015

2020

Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Jia,Jiadong Sun,Wenting Liao,Lingling Qin,Kai Su,Yiqing He,Jiaqiang Zhang,Ronchung Yang,Zhiguang Zhang,Yangpeng Sun

Oncology letters 18:2605-2611 PubMed31404324

2019

Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Chen,Xuming Wang,Limian Song,Dujuan Yao,Qianqian Tang,Jun Zhou

Oncology letters 17:2923-2930 PubMed30854069

2019

Long non-coding RNA-OIS1 inhibits HPV-positive, but not HPV-negative cervical squamous cell carcinoma by upregulating MTK-1.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhou,Fengli Wu,Ying Cui,Fenghua Wei,Qingwei Meng,Qiubo Lv

Oncology letters 16:3453-3458 PubMed30127948

2018

Downregulation of ERBB3 decreases the proliferation, migration and invasion of cervical cancer cells though the interaction with MTK-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyun Du,Shihua Zhou,Li Wang,Mulan Yu,Liyan Mei

Nature structural & molecular biology 22:759-66 PubMed26323038

2015

Histone monoubiquitination by Clock-Bmal1 complex marks Per1 and Per2 genes for circadian feedback.

Applications

Species

Mouse

Alfred G Tamayo,Hao A Duong,Maria S Robles,Matthias Mann,Charles J Weitz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP3K4 antibody

Immunoprecipitation - Anti-MAP3K4 antibody (AB186125)

Western blot - Anti-MAP3K4 antibody (AB186125)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise