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AB308011

Anti-MAP3K8/COT antibody [RP23040075]

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Anti-MAP3K8/COT antibody [RP23040075] is a Rabbit Recombinant Multiclonal antibody that is used in MAP3K8/COT ICC/IF, Western Blot. Suitable for Human, Mouse samples.

MAP3K8 is protein kinase which activated by phosphorylation during the S and G2/M phases of the cell cycle. MAP3K8 has been identified as an oncogene and can activate both MAP kinase and JNLK kinase pathways.

View Alternative Names

COT, ESTF, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

Immunofluorescence analysis of 4% paraformaldehyde on 70% confluent log phase U-87MG cells  labelling COT/MAP3K8 with ab308011 at 2 µg/mL, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1 : 300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)
  • WB

Supplier Data

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

Western blot analysis of MAP3K8/COT in whole cell extracts from Jurkat cells (lane 1), 3T3L1 cells (lane 2), and HepG2 cells (lane 3) using ab308011 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.

All lanes:

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (ab308011) at 2 µg/mL

Lane 1:

Jurkat cells whole cell lysate

Lane 2:

3T3L1 whole cell lysate

Lane 3:

HepG2 whole cell lysate

Observed band size: 52 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040075

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human MAP3K8.

P41279

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
See full target information MAP3K8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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