AB228752

Anti-MAP3K9 antibody

Name: Anti-MAP3K9 antibody (ab228752) | Abcam
Brand: Abcam Limited
SKU: AB228752

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(6 Publications)

Rabbit Polyclonal MAP3K9 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K9.

View Alternative Names

MLK1, PRKE1, MAP3K9, Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (AB228752)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (AB228752)

4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for MAP3K9 (green) using ab228752 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-MAP3K9 antibody (AB228752)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MAP3K9 antibody (ab228752) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

Lane 3:

NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 122 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAP3K9. The exact immunogen used to generate this antibody is proprietary information.

P80192

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Also plays a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

See full target information MAP3K9

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:5539-5550 PubMed39659936

2024

LncRNA SLC16A1-AS1 participates in the initiation and progression of colorectal cancer by regulating MAP3K9 expression through targeting miR-515-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Wanxing Xu,Suzhen Bi,Meichun Xing

Aging 16:7915-7927 PubMed38728237

2024

IL21 inhibits miR-361-5p to promote MAP3K9 and further aggravate the progression of shoulder arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kangning Hao,Pengchao Lin,Jing Li,Jie Hu,Jiangyong Wang,Fei Li

Biology 10: PubMed34439974

2021

Investigation of Anti-Tumor Effects of an MLK1 Inhibitor in Prostate and Pancreatic Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ching Fan,Kai-Cheng Hsu,Tony-Eight Lin,Dietmar Zechner,Sung-Po Hsu,Yuan-Chin Tsai

Experimental and therapeutic medicine 21:574 PubMed33850546

2021

MicroRNA-361-5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbo Ren,Xiangmei Xiao,Xiaoqian Leng,Qi Zhang,Xue Zhou,Ziyuan Ren,Hang Xiao

Cancer biotherapy & radiopharmaceuticals : PubMed32897735

2020

Circ_SIPA1L1 Promotes Osteosarcoma Progression Via miR-379-5p/MAP3K9 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Jun,Li Xuhong,Liu Hui

International journal of molecular medicine 44:240-252 PubMed31115491

2019

miR‑490‑5p regulates the proliferation, migration, invasion and epithelial‑mesenchymal transition of pharyngolaryngeal cancer cells by targeting mitogen‑activated protein kinase kinasekinase 9.

Applications

Unspecified application

Species

Unspecified reactive species

Arikin Abdeyrim,Xiuqin Cheng,Meng Lian,Yuanyouan Tan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP3K9 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K9 antibody (AB228752)

Western blot - Anti-MAP3K9 antibody (AB228752)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise