AB224543

Anti-MAP4 antibody

Name: Anti-MAP4 antibody (ab224543) | Abcam
Brand: Abcam Limited
SKU: AB224543
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP4 aa 550-650.

View Alternative Names

Microtubule-associated protein 4, MAP-4, MAP4

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Paraffin-embedded human skeletal muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Paraffin-embedded human testis tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Paraffin-embedded human kidney stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MAP4 antibody (AB224543)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP4 antibody (AB224543)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MAP4 (green) using ab224543 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Paraffin-embedded human soft tissue tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Paraffin-embedded human heart muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MAP4 antibody (AB224543)

All lanes:

Western blot - Anti-MAP4 antibody (ab224543) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAP4 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

P27816

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

See full target information MAP4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pediatrics 11:1160107 PubMed37215601

2023

Case report: Muscle involvement in a Chinese patient with -related disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Jie Wei,Yi-Dan Liu,Yan-Ling Yang,Yuan Wu,Jie-Yu Liu,Xing-Zhi Chang,Ying Hua,Yu-He Liu,Hui Xiong

Cell reports 38:110305 PubMed35081344

2022

Compartmentalized control of Cdk1 drives mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Flavia Serpico,Francesco Febbraro,Caterina Pisauro,Domenico Grieco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Immunocytochemistry/ Immunofluorescence - Anti-MAP4 antibody (AB224543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB224543)

Western blot - Anti-MAP4 antibody (AB224543)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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