Rabbit Polyclonal MAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAP4 aa 550-650.
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Publications
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- Cell reports 38:1103052022Compartmentalized control of Cdk1 drives mitotic spindle assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela Flavia Serpico,Francesco Febbraro,Caterina Pisauro,Domenico GriecoPubMed 35081344
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAP4 aa 550-650. The exact immunogen used to generate this antibody is proprietary information. Database link P27816
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Non-neuronal microtubule-associated protein. Promotes microtubule assembly.
Alternative names
Microtubule-associated protein 4, MAP-4, MAP4
Recommended products
Rabbit Polyclonal MAP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAP4 aa 550-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAP4 aa 550-650. The exact immunogen used to generate this antibody is proprietary information. Database link P27816
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab224543)
Paraffin-embedded human kidney stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab224543)
Paraffin-embedded human skeletal muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab224543)
Paraffin-embedded human soft tissue tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab224543)
Paraffin-embedded human testis tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-MAP4 antibody (ab224543)
All lanes: Western blot - Anti-MAP4 antibody (ab224543) at 1/100 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Predicted band size: 121 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab224543)
Paraffin-embedded human heart muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MAP4 antibody (ab224543)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MAP4 (green) using ab224543 at 4 μg/ml in ICC/IF.
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