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AB129319

Anti-MAP4K1/HPK1 antibody

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(2 Publications)

Goat Polyclonal MAP4K1/HPK1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAP4K1 aa 1-50.

View Alternative Names

HPK1, MAP4K1, Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1

2 Images
Western blot - Anti-MAP4K1/HPK1 antibody (AB129319)
  • WB

Unknown

Western blot - Anti-MAP4K1/HPK1 antibody (AB129319)

All lanes:

Western blot - Anti-MAP4K1/HPK1 antibody (ab129319) at 0.5 µg/mL

All lanes:

Jurkat cell lysate in RIPA buffer at 35 µg

Predicted band size: 91 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (AB129319)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody (AB129319)

ab129319 at 3.75 μg/ml staining MAP4K1/HPK1 in Formalin-Fixed, Paraffin-Embedded Human thymus tissue by Immunohistochemistry.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MAP4K1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q92918

Specificity

ab129319 is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase, which plays a role in the response to environmental stress (PubMed : 24362026). Appears to act upstream of the JUN N-terminal pathway (PubMed : 8824585). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). May play a role in hematopoietic lineage decisions and growth regulation (PubMed : 24362026, PubMed : 8824585). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).
See full target information MAP4K1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 21:117 PubMed33832481

2021

POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1.

Applications

Unspecified application

Species

Unspecified reactive species

Ronggang Luo,Yi Zhuo,Quan Du,Rendong Xiao

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:7037-7048 PubMed30870006

2019

Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Lei,Hui-Qing Li,Zhi-Huai Mo,Ke-Jia Liu,Ling-Juan Zhu,Chun-Yi Li,Wen-Li Chen,Lei Zhang
View all publications
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