AB69885

Anti-MAP4K4/NIK antibody

Name: Anti-MAP4K4/NIK antibody (ab69885) | Abcam
Brand: Abcam Limited
SKU: AB69885

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(1 Publication)

Rabbit Polyclonal MAP4K4/NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP4K4.

View Alternative Names

HGK, KIAA0687, NIK, MAP4K4, Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, Nck-interacting kinase, MEK kinase kinase 4, MEKKK 4

1 Images

Unknown

Western blot - Anti-MAP4K4/NIK antibody (AB69885)

All lanes:

Western blot - Anti-MAP4K4/NIK antibody (ab69885) at 1/500 dilution

Lane 1:

Extracts from COLO cells at 5 µg

Lane 2:

Extracts from HUVEC cells at 5 µg

Lane 3:

Extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 142 kDa

Observed band size: 142 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAP4K4. The exact immunogen used to generate this antibody is proprietary information.

O95819

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). Phosphorylates SMAD1 on Thr-322 (PubMed : 21690388).

See full target information MAP4K4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 27:1387-95 PubMed21945944

2011

Urinary organic anion transporter protein profiles in AKI.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Kunin,Eli J Holtzman,Semyon Melnikov,Dganit Dinour

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP4K4/NIK antibody

Western blot - Anti-MAP4K4/NIK antibody (AB69885)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise