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AB150300

Anti-MAP4K4/NIK antibody [SP177]

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(1 Publication)

Rabbit Recombinant Monoclonal MAP4K4/NIK antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HGK, KIAA0687, NIK, MAP4K4, Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, Nck-interacting kinase, MEK kinase kinase 4, MEKKK 4

2 Images
Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (AB150300)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (AB150300)

Flow cytometric analysis of rabbit anti-MAP4K4/NIK (SP177) antibody ab150300 (1/400) in HepG2 cells(green) compared to negative control of rabbit IgG (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody [SP177] (AB150300)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody [SP177] (AB150300)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate adenocarcinoma tissue labelling MAP4K4/NIK with ab150300 at 1/100 dilution.

  • Carrier free

    Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP177

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). Phosphorylates SMAD1 on Thr-322 (PubMed : 21690388).
See full target information MAP4K4

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta diabetologica 62:1403-1416 PubMed39945887

2025

The UBR5 protein facilitates mesangial cell hypertrophy and glycolysis induced by high glucose by increasing the phosphorylation levels of AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Liao,Qiming Xu,Jie Xu,Jie Chen,Wenrui Liu,Wenhao Chen,Yunqing Tang,Lianxiang Duan,Yue Guo,Ziyang Liu,Pengyu Tao,Yu Cao,Jianrao Lu,Jing Hu
View all publications

Product promise

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For full details, please see our Terms & Conditions

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