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AB96551

Anti-MAP4K5 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal MAP4K5 antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAP4K5 aa 400-500.

View Alternative Names

Mitogen-activated protein kinase kinase kinase kinase 5, Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, KHS, MEK kinase kinase 5, MEKKK 5, MAP4K5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP4K5 antibody (AB96551)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAP4K5 antibody (AB96551)

ab96551, at 1/200 dilution, staining MAP4K5 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K5 antibody (AB96551)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K5 antibody (AB96551)

ab96551, at 1/100 dilution, staining MAP4K5 in paraffin-embedded SAS xenograft by Immunohistochemistry.

Western blot - Anti-MAP4K5 antibody (AB96551)
  • WB

Unknown

Western blot - Anti-MAP4K5 antibody (AB96551)

12% SDS PAGE

All lanes:

Western blot - Anti-MAP4K5 antibody (ab96551) at 1/5000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 95 kDa

false

Immunoprecipitation - Anti-MAP4K5 antibody (AB96551)
  • IP

Supplier Data

Immunoprecipitation - Anti-MAP4K5 antibody (AB96551)

Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 µg of ab96551.
Western blot analysis was performed using ab96551.

All lanes:

Immunoprecipitation - Anti-MAP4K5 antibody (ab96551) at 5 µg

Lane 1:

293T whole cell extracts

Lane 2:

293T whole cell extracts with control IgG

Lane 3:

Immunoprecipitated 293T whole cell extracts using ab96551

Secondary

All lanes:

Anti Rabbit IgG

Predicted band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human MAP4K5 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4K4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
See full target information MAP4K5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 220: PubMed36820830

2023

Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Mitchell J Geer,Xinyi Guo,Igor Dolgalev,Neville E Sanjana,Benjamin G Neel

Redox biology 34:101539 PubMed32353747

2020

Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Mota-Martorell,Mariona Jove,Irene Pradas,Isabel Sanchez,José Gómez,Alba Naudi,Gustavo Barja,Reinald Pamplona
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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