Rabbit Recombinant Monoclonal MAPK6/ERK3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--western-blot-img620.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348)")
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--western-blot-img141433.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101448.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (AB247348)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
Alternative names
ERK3, PRKM6, MAPK6, Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, MAP kinase isoform p97, ERK-3, p97-MAPK
Recommended products
Rabbit Recombinant Monoclonal MAPK6/ERK3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1720Y
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247348 is the carrier-free version of Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--western-blot-img620.jpg "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)")
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277).
Lanes 1-3: Merged signal (red and green). Green - Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 Anti-MAPK6/ERK3 antibody [EP1720Y] was shown to specifically react with 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MAPK6 (ERK3) knockout HeLa cell line ab264910 (knockout cell lysate Human MAPK6 (ERK3) knockout HeLa cell lysate ab257526) was used. Wild-type and 3 knockout samples were subjected to SDS-PAGE. Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MAPK6 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MAPK6 (ERK3) knockout HeLa cell line (Human MAPK6 (ERK3) knockout HeLa cell line ab264910)
Lane 3: 2.4G2 (Rat B cell lymphoma B lymphocyte) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--western-blot-img141433.jpg "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)")
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 was shown to react with MAPK6 in HAP1 wild-type cells in Western blot. Loss of signal was observed when MAPK6 knockout sample was used. HAP1 wild-type and MAPK6 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: MAPK6 knockout HAP1 whole cell lysate at 20 µg
Lane 3: A431 whole cell lysate at 20 µg
Lane 4: MCF7 whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348), expandable thumbnail](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-bsa-and-azide-free-ab247348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101448.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] - BSA and Azide free (ab247348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling MAPK6/ERK3 with Purified Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 at 1:100 dilution (13.52 μg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277)
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