AB74032

Anti-MAPK6/ERK3 (phospho S189) antibody

Name: Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032) | Abcam
Brand: Abcam Limited
SKU: AB74032
Price: 535 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal MAPK6/ERK3 phospho S189 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK6 pS189.

View Alternative Names

ERK3, PRKM6, MAPK6, Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, MAP kinase isoform p97, ERK-3, p97-MAPK

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab74032, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

Unknown

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

All lanes:

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032) at 1/500 dilution

Lane 1:

Extracts from mouse brain at 5 µg

Lane 2:

Extracts from mouse brain at 5 µg with immunising phosphopeptide

Predicted band size: 83 kDa

Observed band size: 90 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MAPK6 pS189. The exact immunogen used to generate this antibody is proprietary information.

Q16659

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab74032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events : upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

See full target information MAPK6 pS189

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 96:102134 PubMed40189102

2025

C3aR1 on β cells enhances β cell function and survival to maintain glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Renan Pereira de Lima,Ang Li,Ankit Gilani,Alfonso Rubio-Navarro,Charles D Warren,Isabella Y Kong,Jacob B Geri,James C Lo

Cell death & disease 13:500 PubMed35614037

2022

Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Brandolini,Michele d'Angelo,Rubina Novelli,Vanessa Castelli,Cristina Giorgio,Anna Sirico,Pasquale Cocchiaro,Francesco D'Egidio,Elisabetta Benedetti,Claudia Cristiano,Antonella Bugatti,Anna Ruocco,Pier Giorgio Amendola,Carmine Talarico,Candida Manelfi,Daniela Iaconis,Andrea Beccari,Andreza U Quadros,Thiago M Cunha,Arnaldo Caruso,Roberto Russo,Annamaria Cimini,Andrea Aramini,Marcello Allegretti

The European journal of neuroscience 55:1471-1482 PubMed35243702

2022

Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Huang,Pei Wang,Haijun Liu,Mingjian Li,Yujiao Yue,Ping Xu

American journal of physiology. Cell physiology : PubMed33502949

2021

Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ping Guo,Zhi-Feng Wang,Na Li,Qian-Qian Lei,Qiong Cheng,Li-Gang Shi,Sheng-Li Zhou,Xin-Hua Wang,Yang Sun,Ling-Fei Kong

Cell cycle (Georgetown, Tex.) 19:1022-1035 PubMed32208888

2020

Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Guangying Li,Longhai Xiao,Hao Qin,Qiang Zhuang,Weiwei Zhang,Long Liu,Chao Di,Yabo Zhang

Bioscience reports 40: PubMed32202300

2020

MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jia,Yipeng Ren,Fengze Wang,Rui Zhao,Bo Qiao,Lejun Xing,Long Ou,Bin Guo

Cell cycle (Georgetown, Tex.) 18:3404-3419 PubMed31650885

2019

Runt-related transcription factor 1 contributes to lung cancer development by binding to tartrate-resistant acid phosphatase 5.

Applications

Unspecified application

Species

Unspecified reactive species

Changjun He,Xue Bai,Yingbin Li,Haobo Sun,Xianglong Kong,Bicheng Fu,Lantao Chen,Kaibin Zhu,Pengju Li,Shidong Xu

Archives of oral biology 102:164-170 PubMed31055249

2019

Over-expression of Bcl2-associated athanogene 2 in oral cancer promotes cellular proliferation and is associated with poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Song Liu,Bing Wei

Journal of hematology & oncology 10:59 PubMed28241849

2017

Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Sunbin Ling,Haiyang Xie,Fan Yang,Qiaonan Shan,Haojiang Dai,Jianyong Zhuo,Xuyong Wei,Penghong Song,Lin Zhou,Xiao Xu,Shusen Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAPK6/ERK3 (phospho S189) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise