AB232970

Anti-MAPRE1/EB1 antibody

Name: Anti-MAPRE1/EB1 antibody (ab232970) | Abcam
Brand: Abcam Limited
SKU: AB232970
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MAPRE1/EB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment - Human, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAPRE1.

View Alternative Names

Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (AB232970)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (AB232970)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MAPRE1/EB1 using ab232970 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (AB232970)

Formalin-fixed, paraffin-embedded human liver tissue stained for MAPRE1/EB1 using ab232970 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB232970)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB232970)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MAPRE1/EB1 (green) using ab232970 at 20 μg/ml in ICC/IF. FITC labeling.

Supplier Data

Western blot - Anti-MAPRE1/EB1 antibody (AB232970)

All lanes:

Western blot - Anti-MAPRE1/EB1 antibody (ab232970) at 3 µg/mL

All lanes:

Recombinant human MAPRE1/EB1 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 30 kDa

false

Unknown

Western blot - Anti-MAPRE1/EB1 antibody (AB232970)

All lanes:

Western blot - Anti-MAPRE1/EB1 antibody (ab232970) at 3 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 3:

Pig brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MAPRE1.

Q15691

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 23001180, PubMed : 28726242, PubMed : 28814570, PubMed : 34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed : 19632184, PubMed : 36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 28726242, PubMed : 28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed : 12388762, PubMed : 34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed : 27225956, PubMed : 36592928). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed : 28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). May play a role in cell migration (By similarity).

See full target information MAPRE1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 82:1181-1190 PubMed37147113

2023

Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B.

Applications

Unspecified application

Species

Unspecified reactive species

Sherri Longobardi,Charmaine Lopez-Davis,Bhuwan Khatri,Constantin Georgescu,Cherilyn Pritchett-Frazee,Christina Lawrence,Astrid Rasmussen,Lida Radfar,Robert Hal Scofield,Alan N Baer,Susan A Robinson,Erika Darrah,Robert C Axtell,Gabriel Pardo,Jonathan D Wren,Kristi A Koelsch,Joel M Guthridge,Judith A James,Christopher J Lessard,Amy Darise Farris

Frontiers in microbiology 12:615165 PubMed33717005

2021

The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lien De Caluwé,Sandra Coppens,Katleen Vereecken,Simon Daled,Maarten Dhaenens,Xaveer Van Ostade,Dieter Deforce,Kevin K Ariën,Koen Bartholomeeusen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAPRE1/EB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (AB232970)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (AB232970)

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB232970)

Western blot - Anti-MAPRE1/EB1 antibody (AB232970)

Western blot - Anti-MAPRE1/EB1 antibody (AB232970)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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