Rabbit Polyclonal MAPRE1/EB1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Bioengineered 13:1814-18272021Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibin Zhang,Hao Peng,Zheyuan Xu,Qiuju Yang,Yang Wang,Han Wang,Liang BuPubMed 34709968
- Experimental cell research 362:477-4882017Functional role for stable microtubules in lens fiber cell elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaitlin M Logan,Caitlin J Bowen,A Sue MenkoPubMed 29253534
- Scientific reports 7:148942017Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvangelina Almada et. al.PubMed 29097729
- Journal of cell science 126:4000-142013The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeborah A Goldspink et. al.PubMed 23813963
- Proceedings of the National Academy of Sciences of 110:3399-4042013Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.
- PloS one 7:e514422012Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.
- PloS one 6:e288842011EB1 is required for spindle symmetry in mammalian mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnke Brüning-Richardson et. al.PubMed 22216133
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15691
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Tested |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed:19632184, PubMed:36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed:27225956, PubMed:36592928). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). May play a role in cell migration (By similarity).
Alternative names
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
Recommended products
Rabbit Polyclonal MAPRE1/EB1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15691
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab50188 is affinity-purified using the immunizing peptide immobilized on agarose.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)
ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol.
The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
Western blot - Anti-MAPRE1/EB1 antibody (ab50188)
50kDa band represents Rb IgG heavy chain
All lanes: Western blot - Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/mL
Lane 1: HeLa cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Rat1 cell lysate
Lane 4: A431 cell lysate
Lane 5: SW48 cell lysate
Lane 6: HCT116 cell lysate
Lane 7: HEK293 cell lysate
Lane 8: HeLa cell lysate with EB1 immunizing peptide
SecondaryAll lanes: Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa, 50 kDa
Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)
RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188.
50kDa band represents Rb IgG heavy chainLane 1: No primary antibody
Lanes 2 - 4: Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg
Lanes 1 - 2: No lysate
Lane 3: HeLa cell lysate at 100 µg
Lane 4: HeLa cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa, 50 kDa
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