AB50188

Anti-MAPRE1/EB1 antibody

Name: Anti-MAPRE1/EB1 antibody (ab50188) | Abcam
Brand: Abcam Limited
SKU: AB50188
Rating: 4 (3 reviews)

(3 Reviews)

(7 Publications)

Rabbit Polyclonal MAPRE1/EB1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1

3 Images

Unknown

Immunoprecipitation - Anti-MAPRE1/EB1 antibody (AB50188)

RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain

Lane 1:

No primary antibody

Lanes 2 - 4:

Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg

Lanes 1 - 2:

No lysate

Lane 3:

HeLa cell lysate at 100 µg

Lane 4:

HeLa cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 34 kDa,50 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB50188)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB50188)

ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1 : 1,000.

Unknown

Western blot - Anti-MAPRE1/EB1 antibody (AB50188)

50kDa band represents Rb IgG heavy chain

All lanes:

Western blot - Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

NIH3T3 cell lysate

Lane 3:

Rat1 cell lysate

Lane 4:

A431 cell lysate

Lane 5:

SW48 cell lysate

Lane 6:

HCT116 cell lysate

Lane 7:

HEK293 cell lysate

Lane 8:

HeLa cell lysate with EB1 immunizing peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 34 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human MAPRE1 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15691

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10-20 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab50188 is affinity-purified using the immunizing peptide immobilized on agarose.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 23001180, PubMed : 28726242, PubMed : 28814570, PubMed : 34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed : 19632184, PubMed : 36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 28726242, PubMed : 28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed : 12388762, PubMed : 34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed : 27225956, PubMed : 36592928). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed : 28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). May play a role in cell migration (By similarity).

See full target information MAPRE1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:1814-1827 PubMed34709968

2021

Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1.

Applications

Unspecified application

Species

Unspecified reactive species

Libin Zhang,Hao Peng,Zheyuan Xu,Qiuju Yang,Yang Wang,Han Wang,Liang Bu

Experimental cell research 362:477-488 PubMed29253534

2017

Functional role for stable microtubules in lens fiber cell elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin M Logan,Caitlin J Bowen,A Sue Menko

Scientific reports 7:14894 PubMed29097729

2017

Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Evangelina Almada,Facundo M Tonucci,Florencia Hidalgo,Anabela Ferretti,Solange Ibarra,Alejandro Pariani,Rodrigo Vena,Cristián Favre,Javier Girardini,Arlinet Kierbel,M Cecilia Larocca

Journal of cell science 126:4000-14 PubMed23813963

2013

The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Deborah A Goldspink,Jonathan R Gadsby,Gemma Bellett,Jennifer Keynton,Benjamin J Tyrrell,Elizabeth K Lund,Penny P Powell,Paul Thomas,Mette M Mogensen

Proceedings of the National Academy of Sciences of 110:3399-404 PubMed23401517

2013

Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.

Applications

ICC/IF

Species

Human

Togo Shimozawa,Kazuo Yamagata,Takefumi Kondo,Shigeo Hayashi,Atsunori Shitamukai,Daijiro Konno,Fumio Matsuzaki,Jun Takayama,Shuichi Onami,Hiroshi Nakayama,Yasuhito Kosugi,Tomonobu M Watanabe,Katsumasa Fujita,Yuko Mimori-Kiyosue

PloS one 7:e51442 PubMed23251535

2012

Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.

Applications

Species

Human

Satoko Nakamura,Ilya Grigoriev,Taisaku Nogi,Tomoko Hamaji,Lynne Cassimeris,Yuko Mimori-Kiyosue

PloS one 6:e28884 PubMed22216133

2011

EB1 is required for spindle symmetry in mammalian mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anke Brüning-Richardson,Kelly J Langford,Peter Ruane,Tracy Lee,Jon M Askham,Ewan E Morrison

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAPRE1/EB1 antibody

Immunoprecipitation - Anti-MAPRE1/EB1 antibody (AB50188)

Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (AB50188)

Western blot - Anti-MAPRE1/EB1 antibody (AB50188)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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