AB53358

Anti-MAPRE1/EB1 antibody [KT51]

Name: Anti-MAPRE1/EB1 antibody [KT51] (ab53358) | Abcam
Brand: Abcam Limited
SKU: AB53358
Price: 565 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(29 Publications)

Rat Monoclonal MAPRE1/EB1 antibody. Suitable for ICC, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Hamster, Human samples. Cited in 29 publications.

View Alternative Names

Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1

1 Images

Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (AB53358)

Flow Cyt

Unknown

Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (AB53358)

Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KT51

Isotype

IgG2a

Carrier free

Reacts with

Human, Hamster, Mouse

Applications

ICC, WB, IP, Flow Cyt, ICC/IF, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 23001180, PubMed : 28726242, PubMed : 28814570, PubMed : 34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed : 19632184, PubMed : 36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed : 12388762, PubMed : 16109370, PubMed : 19632184, PubMed : 21646404, PubMed : 28726242, PubMed : 28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed : 12388762, PubMed : 34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed : 27225956, PubMed : 36592928). Also acts as a regulator of minus-end microtubule organization : interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed : 28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed : 28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). May play a role in cell migration (By similarity).

See full target information MAPRE1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1582924 PubMed40546321

2025

Hedgehog signaling controls astral microtubules and mitotic spindle orientation in neural progenitors and iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Fengming Liu,Anna Medyukhina,Kris M Olesen,Abbas Shirinifard,Hongjian Jin,Lei Li,Marina Mapelli,Khaled Khairy,Young-Goo Han

Acta neuropathologica communications 12:62 PubMed38637827

2024

ADNP dysregulates methylation and mitochondrial gene expression in the cerebellum of a Helsmoortel-Van der Aa syndrome autopsy case.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio D'Incal,Anke Van Dijck,Joe Ibrahim,Kevin De Man,Lina Bastini,Anthony Konings,Ellen Elinck,Claudia Theys,Illana Gozes,Zlatko Marusic,Mirna Anicic,Jurica Vukovic,Nathalie Van der Aa,Ligia Mateiu,Wim Vanden Berghe,R Frank Kooy

Nature cell biology 26:404-420 PubMed38424271

2024

CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC.

Applications

Unspecified application

Species

Unspecified reactive species

Dipti Rai,Yinlong Song,Shasha Hua,Kelly Stecker,Jooske L Monster,Victor Yin,Riccardo Stucchi,Yixin Xu,Yaqian Zhang,Fangrui Chen,Eugene A Katrukha,Maarten Altelaar,Albert J R Heck,Michal Wieczorek,Kai Jiang,Anna Akhmanova

The Journal of general physiology 156: PubMed38226948

2024

Persistent PKA activation redistributes NaV1.5 to the cell surface of adult rat ventricular myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tytus Bernas,John Seo,Zachary T Wilson,Bi-Hua Tan,Isabelle Deschenes,Christiane Carter,Jinze Liu,Gea-Ny Tseng

The Journal of biological chemistry 299:102853 PubMed36592928

2023

Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1.

Applications

Unspecified application

Species

Unspecified reactive species

Renjith M Radhakrishnan,Safwa T Kizhakkeduth,Vishnu M Nair,Shine Ayyappan,R Bhagya Lakshmi,Neethu Babu,Anjaly Prasannajith,Kenichi Umeda,Vinesh Vijayan,Noriyuki Kodera,Tapas K Manna

Frontiers in cellular neuroscience 16:989945 PubMed36385945

2022

Decreased tubulin-binding cofactor B was involved in the formation disorder of nascent astrocyte processes by regulating microtubule plus-end growth through binding with end-binding proteins 1 and 3 after chronic alcohol exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Zheng,Mei Yang,Xiaoqiao Chen,Gaoli Zhang,Shanshan Wan,Bingqiu Zhang,Jiechao Huo,Hui Liu

Cell reports 39:110610 PubMed35385739

2022

Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Ana C Almeida,Joana Soares-de-Oliveira,Danica Drpic,Liam P Cheeseman,Joana Damas,Harris A Lewin,Denis M Larkin,Paulo Aguiar,António J Pereira,Helder Maiato

Human molecular genetics 31:2639-2654 PubMed35333353

2022

XBP1 variant 1 promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6.

Applications

Unspecified application

Species

Unspecified reactive species

Yongwang Zhong,Wenjing Yan,Jingjing Ruan,Mike Fang,Changjun Yu,Shaojun Du,Ganesha Rai,Dingyin Tao,Mark J Henderson,Shengyun Fang

Developmental cell 56:3264-3275.e7 PubMed34672971

2021

CLIP-170S is a microtubule +TIP variant that confers resistance to taxanes by impairing drug-target engagement.

Applications

Unspecified application

Species

Unspecified reactive species

Prashant V Thakkar,Katsuhiro Kita,Urko Del Castillo,Giuseppe Galletti,Neel Madhukar,Elena Vila Navarro,Isabel Barasoain,Holly V Goodson,Dan Sackett,José Fernando Díaz,Yao Lu,Arindam RoyChoudhury,Henrik Molina,Olivier Elemento,Manish A Shah,Paraskevi Giannakakou

Journal of neurochemistry 160:185-202 PubMed34478582

2021

The drebrin/EB3 pathway regulates cytoskeletal dynamics to drive neuritogenesis in embryonic cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Thanushiyan Poobalasingam,Francesca Bianco,Fazal Oozeer,Phillip R Gordon-Weeks

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAPRE1/EB1 antibody [KT51]

Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (AB53358)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (29)

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