Rat Monoclonal MAPRE1/EB1 antibody. Suitable for ICC, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Hamster, Human samples. Cited in 23 publications.
IgG2a
Rat
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Monoclonal
ICC | IP | Flow Cyt | ELISA | WB | ICC/IF | |
---|---|---|---|---|---|---|
Human | Expected | Expected | Tested | Expected | Expected | Expected |
Mouse | Expected | Expected | Predicted | Expected | Expected | Expected |
Hamster | Expected | Expected | Predicted | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Hamster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Hamster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Hamster, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Hamster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Hamster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Hamster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293). Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains (PubMed:19632184, PubMed:36592928). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293). Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers) (PubMed:27225956, PubMed:36592928). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (By similarity). May play a role in cell migration (By similarity).
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
Rat Monoclonal MAPRE1/EB1 antibody. Suitable for ICC, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Hamster, Human samples. Cited in 23 publications.
IgG2a
Rat
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Monoclonal
KT51
Affinity purification Protein G
Blue Ice
Up to 12 months
+4°C
-20°C
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Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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