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AB275632

Anti-Marapsin1 antibody [107]

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Rabbit Recombinant Monoclonal Marapsin1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRSS27.

View Alternative Names

MPN, UNQ1884/PRO4327, PRSS27, Serine protease 27, Marapsin, Pancreasin

2 Images
Immunoprecipitation - Anti-Marapsin1 antibody [107] (AB275632)
  • IP

Unknown

Immunoprecipitation - Anti-Marapsin1 antibody [107] (AB275632)

Marapsin1 was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate with 2 μL of ab275632 and 15 μL of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab275632 at 1/200 dilution. Rabbit IgG (H+L) Dylight 800-labeled, was used as secondary antibody at 1/5000 dilution.

Lane 1 : ab275632 IP in K562 lysate

Developed using the Odssey technique.

All lanes:

Immunoprecipitation - Anti-Marapsin1 antibody [107] (ab275632)

Predicted band size: 32 kDa

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Western blot - Anti-Marapsin1 antibody [107] (AB275632)
  • WB

Supplier Data

Western blot - Anti-Marapsin1 antibody [107] (AB275632)

Developed using the Odyssey technique.

All lanes:

Western blot - Anti-Marapsin1 antibody [107] (ab275632) at 1/500 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

107

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRSS27.

Q9BQR3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>4-6 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Marapsin1 also known as PRSS27 is a serine protease with a molecular mass of approximately 25 kDa. It functions by cleaving peptide bonds in substrates which is typical for enzymes belonging to its class. Expression of Marapsin1 occurs mainly in epithelial tissues especially in the skin and some glandular tissues like the pancreas. Its role is linked to various cellular mechanisms dependent on proteolytic processes.
Biological function summary

This protease contributes to essential cellular processes such as keratinization and epidermal differentiation. It is not part of any large multiprotein complexes but interacts closely with substrates involved in skin barrier formation. The specificity of Marapsin1’s substrate recognition plays a significant role in maintaining the integrity and proper function of epithelial tissues.

Pathways

Marapsin1 is involved in the epidermal differentiation pathway which is important for skin health and regeneration. This pathway includes other proteins such as filaggrin and loricrin which are important for skin barrier formation. Marapsin1's precise proteolytic actions help modulate the availability and activity of these proteins thereby ensuring proper skin structure and resilience against external stress.

Disrupted Marapsin1 activity is associated with skin conditions particularly psoriasis and atopic dermatitis. These conditions often involve altered epidermal differentiation processes. Marapsin1's interaction with proteins like filaggrin suggests its significance in maintaining healthy skin. Abnormalities in these interactions can lead to compromised skin integrity triggering or exacerbating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PRSS27
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