Skip to main content

Rabbit Recombinant Monoclonal MARC1 antibody. Suitable for WB, Flow Cyt (Intra), IP, IHC-P, ICC/IF, mIHC and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MARC1 antibody [EPR28272-93] (AB317262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (AB317262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (AB317262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (AB317262), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (AB317262), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IPIHC-PICC/IFmIHC
Human
Tested
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Tested
Expected
Expected
Recombinant full length protein - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Catalyzes the reduction of N-oxygenated molecules, acting as a counterpart of cytochrome P450 and flavin-containing monooxygenases in metabolic cycles (PubMed:19053771, PubMed:21029045, PubMed:30397129). As a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability (PubMed:19053771). May be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis (PubMed:21029045). Postulated to cleave the N-OH bond of N-hydroxylated substrates in concert with electron transfer from NADH to cytochrome b5 reductase then to cytochrome b5, the ultimate electron donor that primes the active site for substrate reduction (PubMed:21029045, PubMed:19053771).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MARC1 antibody. Suitable for WB, Flow Cyt (Intra), IP, IHC-P, ICC/IF, mIHC and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28272-93

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

20 product images

  • Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    This antibody does not cross-react with human MARC2.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, Anti-Flag antibody (1:5000) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262) at 1/1000 dilution

    Lane 1: His-tagged human MARC1 fl length recombinant protein at 10 ng

    Lane 2: Flag-tagged human MARC2 fl length recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 36 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on rat cardiac muscle. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on rat lung. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on mouse cardiac muscle (PMID: 20861021). The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on mouse lung (PMID: 20861021). The section was incubated with ab317262 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling MARC1 with ab317262 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on human lung. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat liver. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver cancer. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARC1 with ab317262 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver (PMID: 20861021). The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling MARC1 with ab317262 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human hepatocellular carcinoma. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MARC1 with ab317262 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human liver. The section was incubated with ab317262 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Low expression: lung, heart (PMID: 20861021).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/2000 dilution.

    All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262) at 1/1000 dilution

    Lane 1: Mouse liver mitochondria fraction at 20 µg

    Lane 2: Mouse liver non-mitochondrial fraction at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse lung tissue lysate at 20 µg

    Lane 5: Mouse heart tissue lysate at 20 µg

    Lane 6: Rat liver tissue lysate at 20 µg

    Lane 7: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 36 kDa, 16 kDa

    Exposure time: 15s

  • Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Low expression: lung, intestine (PMID: 20861021).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20861021).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/2000 dilution.

    All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262) at 1/1000 dilution

    Lane 1: Human liver mitochondria fraction at 20 µg

    Lane 2: Human liver non-mitochondrial fraction at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Lane 4: Human lung tissue lysate at 20 µg

    Lane 5: Human small intestine tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 36 kDa, 16 kDa

    Exposure time: 3s

  • Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Exposure time: Lane 1: 26 seconds; Lane 2: 180 seconds

    All lanes: Western blot - Anti-MARC1 antibody [EPR28272-93] (ab317262) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 36 kDa

  • Flow Cytometry (Intracellular) - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MARC1 with ab317262 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human colon.

    Panel B: anti-MARC1 staining on human colon.

    Panel C: anti-COX IV staining on human colon.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human kidney.

    Panel B: anti-MARC1 staining on several kidney tubules of human kidney.

    Panel C: anti-COX IV staining on kidney tubules of human kidney.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human liver.

    Panel B: anti-MARC1 staining in hepatocyte of human liver.

    Panel C: anti-COX IV staining in hepatocyte of human liver.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MARC1 with ab317262 at 1/50 (10.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing mitochondrial staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/1000 (1ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunoprecipitation - Anti-MARC1 antibody [EPR28272-93] (ab317262), expandable thumbnail

    Immunoprecipitation - Anti-MARC1 antibody [EPR28272-93] (ab317262)

    MARC1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab317262 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317262 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 2: ab317262 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317262 in HepG2 whole cell lysate.

    All lanes: Immunoprecipitation - Anti-MARC1 antibody [EPR28272-93] (ab317262) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 119s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com