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AB137261

Anti-MARCH3 antibody

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(1 Publication)

Rabbit Polyclonal MARCH3 antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MARCHF3 aa 100-200.

View Alternative Names

MARCH3, RNF173, MARCHF3, E3 ubiquitin-protein ligase MARCHF3, Membrane-associated RING finger protein 3, Membrane-associated RING-CH protein III, RING finger protein 173, RING-type E3 ubiquitin transferase MARCHF3, MARCH-III

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCH3 antibody (AB137261)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCH3 antibody (AB137261)

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using ab137261 at 1 : 50 dilution. The picture on the right is blocked with the synthesized peptide.

Western blot - Anti-MARCH3 antibody (AB137261)
  • WB

Supplier Data

Western blot - Anti-MARCH3 antibody (AB137261)

All lanes:

Western blot - Anti-MARCH3 antibody (ab137261) at 1/500 dilution

Lane 1:

K562 cell line

Lane 2:

COS7 cell line

Lane 3:

K562 cell line with Blocking with synthesized peptide

Predicted band size: 29 kDa

false

Western blot - Anti-MARCH3 antibody (AB137261)
  • WB

Unknown

Western blot - Anti-MARCH3 antibody (AB137261)

All lanes:

Western blot - Anti-MARCH3 antibody (ab137261) at 1/500 dilution

All lanes:

Raw264.7 cell lysate at 30 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MARCHF3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q86UD3

Specificity

ab137261 detects endogenous levels of total MARCH3 protein.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab137261 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
See full target information MARCHF3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 15:2015-2022 PubMed28260052

2017

LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Lu,Xueli Yuan,Qiang Zhang,Hong Zhang,Baozhong Shen
View all publications

Product promise

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