Rabbit Recombinant Monoclonal MARCKS like protein antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--western-blot-img57153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55002.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)")
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--western-blot-img51306.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)")
![Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--immunocytochemistry-immunofluorescence-img75551.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)")
![Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--flow-cytometry-intracellular-img81283.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation (PubMed:22751924). When unphosphorylated, induces cell migration (By similarity). When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration (By similarity). May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity).
Alternative names
MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS, MLP, MARCKSL1, MRP
Recommended products
Rabbit Recombinant Monoclonal MARCKS like protein antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Alternative names
MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS, MLP, MARCKSL1, MRP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR15353
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250750 is the carrier-free version of Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--western-blot-img57153.jpg "Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)")
Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)
This data was developed using Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546, the same antibody clone in a different buffer formulation.
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.
All lanes: Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546) at 1/10000 dilution
Lane 1: 293T cell lysate at 20 µg
Lane 2: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55002.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)
This data was developed using Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--western-blot-img51306.jpg "Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)")
Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)
This data was developed using Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546, the same antibody clone in a different buffer formulation.
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.
All lanes: Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546) at 1/50000 dilution
All lanes: Human fetal brain tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--immunocytochemistry-immunofluorescence-img75551.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)")
Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)
This data was developed using Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-bsa-and-azide-free-ab250750--flow-cytometry-intracellular-img81283.jpg "Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)")
Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (ab250750)
This data was developed using Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with Anti-MARCKS like protein antibody [EPR15353] - C-terminal ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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