Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)

This data was developed using ab184546, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)

This data was developed using ab184546, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)

This data was developed using ab184546, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)

This data was developed using ab184546, the same antibody clone in a different buffer formulation.

The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.

All lanes:

Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (<a href='/en-us/products/primary-antibodies/marcks-like-protein-antibody-epr15353-c-terminal-ab184546'>ab184546</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-MARCKS like protein antibody [EPR15353] - BSA and Azide free (AB250750)

This data was developed using ab184546, the same antibody clone in a different buffer formulation.

The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.

All lanes:

Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (<a href='/en-us/products/primary-antibodies/marcks-like-protein-antibody-epr15353-c-terminal-ab184546'>ab184546</a>) at 1/50000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false