Rabbit Recombinant Monoclonal MARCKS like protein antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--western-blot-img57153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55002.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546)")
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--western-blot-img51306.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546)")
![Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--immunocytochemistry-immunofluorescence-img75551.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546)")
![Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--flow-cytometry-intracellular-img81283.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (AB184546)")
Publications
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- Stem cells international 2023:28268152023A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin LiuPubMed 37964983
- STAR protocols 2:1007472021Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth K Neumann,Nathan Heath Patterson,Jamie L Allen,Lukasz G Migas,Haichun Yang,Maya Brewer,David M Anderson,Jennifer Harvey,Danielle B Gutierrez,Raymond C Harris,Mark P deCaestecker,Agnes B Fogo,Raf Van de Plas,Richard M Caprioli,Jeffrey M SpragginsPubMed 34430920
- American journal of physiology. Cell physiology 319:C589-C6042020Myristoylated alanine-rich C kinase substrate-like protein-1 regulates epithelial sodium channel activity in renal distal convoluted tubule cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Song,Qiang Yue,Auriel Moseley,Otor Al-Khalili,Brandi M Wynne,Heping Ma,Lihua Wang,Douglas C EatonPubMed 32639874
- Molecular & cellular proteomics : MCP 18:1732-17442019Cross-linking Proteomics Indicates Effects of Simvastatin on the TLR2 Interactome and Reveals ACTR1A as a Novel Regulator of the TLR2 Signal Cascade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbu Hena Mostafa Kamal et. al.PubMed 31221720
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/140 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation (PubMed:22751924). When unphosphorylated, induces cell migration (By similarity). When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration (By similarity). May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity).
Alternative names
MLP, MRP, MARCKSL1, MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS
Recommended products
Rabbit Recombinant Monoclonal MARCKS like protein antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15353
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--western-blot-img57153.jpg "Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)")
Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation
All lanes: Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/10000 dilution
Lane 1: 293T cell lysate at 20 µg
Lane 2: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55002.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--western-blot-img51306.jpg "Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)")
Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.
All lanes: Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/50000 dilution
All lanes: Human fetal brain tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--immunocytochemistry-immunofluorescence-img75551.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)")
Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546), expandable thumbnail](https://content.abcam.com/products/images/marcks-like-protein-antibody-epr15353-c-terminal-ab184546--flow-cytometry-intracellular-img81283.jpg "Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)")
Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
Intracellular flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like proteinwith ab184546 at 1/140 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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