AB81295

Anti-MARCKS (phospho S158) antibody [EP2113Y]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal MARCKS phospho S158 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 10 publications.

View Alternative Names

MACS, PRKCSL, MARCKS, Myristoylated alanine-rich C-kinase substrate, 80K-L protein, PKCSL

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Supplier Data

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution

Lane 1:

HEK293 cells treated with TPA at 10 µg

Lane 2:

HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 87 kDa

false

Unknown

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

All lanes:

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution

Lane 1:

Hela cell lysates, untreated. at 10 µg

Lane 2:

Hela cell lysates treated with TPA. at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 87 kDa

false

Lab

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution

Lane 1:

Rat brain at 10 µg

Lane 2:

Mouse brain at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 87 kDa

false

Carrier free

Anti-MARCKS (phospho S158) antibody [EP2113Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2113Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes (PubMed : 23704996, PubMed : 36009319). Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis (PubMed : 23704996). During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages (PubMed : 37949888). Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton (By similarity). Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane (By similarity).

See full target information MARCKS phospho S158

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 13:815301 PubMed35418879

2022

Protein Kinase C-Mediated Hyperphosphorylation and Lateralization of Connexin 43 Are Involved in Autoimmune Myocarditis-Induced Prolongation of QRS Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlian Zhong,Huan Zhao,Xinwen Xie,Zhi Qi,Yumei Li,Lee Jia,Jinwei Zhang,Yusheng Lu

Science immunology 7:eabk2092 PubMed35119941

2022

Epithelial Gasdermin D shapes the host-microbial interface by driving mucus layer formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Qianzhou Yu,Danlu Jiang,Kang Yu,Weiwei Yu,Zhexu Chi,Sheng Chen,Mobai Li,Dehang Yang,Zhen Wang,Ting Xu,Xingchen Guo,Kailian Zhang,Hui Fang,Qizhen Ye,Yong He,Xue Zhang,Di Wang

FASEB journal : official publication of the Federa 33:14354-14369 PubMed31661644

2019

Tackling MARCKS-PIP3 circuit attenuates fibroblast activation and fibrosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

David C Yang,Ji-Min Li,Jihao Xu,Justin Oldham,Sem H Phan,Jerold A Last,Reen Wu,Ching-Hsien Chen

British journal of pharmacology 176:1122-1134 PubMed30706455

2019

Targeting the phosphorylation site of myristoylated alanine-rich C kinase substrate alleviates symptoms in a murine model of steroid-resistant asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Neng Wang,Yu-Chao Lin,Bo-Chun Chang,Ching-Hsien Chen,Reen Wu,Chen-Chen Lee

Oncology letters 13:1216-1222 PubMed28454237

2017

MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines.

Applications

Species

Human

Timothy D Rohrbach,Robert B Jones,Patricia H Hicks,Alice N Weaver,Tiffiny S Cooper,Nicholas J Eustace,Eddy S Yang,John S Jarboe,Joshua C Anderson,Christopher D Willey

International journal of oncology 46:1079-88 PubMed25524703

2014

Targeting the effector domain of the myristoylated alanine rich C-kinase substrate enhances lung cancer radiation sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy D Rohrbach,John S Jarboe,Joshua C Anderson,Hoa Q Trummell,Patricia H Hicks,Alice N Weaver,Eddy S Yang,Robert A Oster,Jessy S Deshane,Chad Steele,Gene P Siegal,James A Bonner,Christopher D Willey

Clinical cancer research : an official journal of 18:3030-41 PubMed22619307

2012

MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma.

Applications

Species

Unspecified reactive species

John S Jarboe,Joshua C Anderson,Christine W Duarte,Tapan Mehta,Somaira Nowsheen,Patricia H Hicks,Alexander C Whitley,Timothy D Rohrbach,Raymond O McCubrey,Sherard Chiu,Tamara M Burleson,James A Bonner,G Yancey Gillespie,Eddy S Yang,Christopher D Willey

British journal of cancer 104:673-84 PubMed21266973

2011

Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C.

Applications

Species

Human

M Rumsby,J Schmitt,M Sharrard,G Rodrigues,M Stower,N Maitland

The Journal of biological chemistry 285:18352-63 PubMed20356841

2010

Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex.

Applications

Unspecified application

Species

Unspecified reactive species

Stepan Gambaryan,Anna Kobsar,Natalia Rukoyatkina,Sabine Herterich,Joerg Geiger,Albert Smolenski,Suzanne M Lohmann,Ulrich Walter

Cellular signalling 22:1097-103 PubMed20211725

2010

PhosphoMARCKS drives motility of mouse melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Chen,Susan A Rotenberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MARCKS (phospho S158) antibody [EP2113Y]

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (AB81295)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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