Rabbit Recombinant Monoclonal MARCO antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115714.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822)")
![Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunoprecipitation-img3285.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27144.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822)")
![Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-frozen-sections-img88467.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822)")
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunocytochemistry-immunofluorescence-img81612.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (AB256822)")
Publications
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- JCI insight 7:2022PLG nanoparticles target fibroblasts and MARCO+ monocytes to reverse multiorgan fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Xu,Swati Bhattacharyya,Wenxia Wang,Igal Ifergan,Ming-Yi Alice Chiang Wong,Daniele Procissi,Anjana Yeldandi,Swarna Bale,Roberta Goncalves Marangoni,Craig Horbinski,Stephen D Miller,John VargaPubMed 35104243
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Mouse | Tested | Not recommended | Expected | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
3 products for Alternative Product
1 products for Alternative Version
Target data
Function
Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed:7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
Alternative names
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco
Recommended products
Rabbit Recombinant Monoclonal MARCO antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
Alternative names
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22944-64
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab256822 is the carrier-free version of Anti-MARCO antibody [EPR22944-64] ab239369.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115714.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MARCO with Anti-MARCO antibody [EPR22944-64] ab239369 at 1/500 dilution (1.11 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells of mouse liver is observed. The section was incubated with Anti-MARCO antibody [EPR22944-64] ab239369 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MARCO antibody [EPR22944-64] ab239369).
![Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunoprecipitation-img3285.jpg "Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)")
Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)
MARCO was immunoprecipitated from 0.35 mg mouse spleen lysate 10μg with Anti-MARCO antibody [EPR22944-64] ab239369 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MARCO antibody [EPR22944-64] ab239369 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10μg.
Lane 2: Anti-MARCO antibody [EPR22944-64] ab239369 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MARCO antibody [EPR22944-64] ab239369 in mouse spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MARCO antibody [EPR22944-64] ab239369).
All lanes: Immunoprecipitation - Anti-MARCO antibody [EPR22944-64] (Anti-MARCO antibody [EPR22944-64] ab239369)
Predicted band size: 53 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27144.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MARCO with Anti-MARCO antibody [EPR22944-64] ab239369 at 1/500 dilution (1.11 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on macrophages of mouse spleen (PMID: 12874264, 15494482) is observed. The section was incubated with Anti-MARCO antibody [EPR22944-64] ab239369 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MARCO antibody [EPR22944-64] ab239369).
![Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunohistochemistry-frozen-sections-img88467.jpg "Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)")
Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)
4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse spleen tissue labeling MARCO using Anti-MARCO antibody [EPR22944-64] ab239369 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on mouse splenic marginal zone (PMID: 24670793) is observed.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MARCO antibody [EPR22944-64] ab239369).
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr22944-64-bsa-and-azide-free-ab256822--immunocytochemistry-immunofluorescence-img81612.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)")
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-64] - BSA and Azide free (ab256822)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J744A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labeling MARCO with Anti-MARCO antibody [EPR22944-64] ab239369 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in J744A.1 cells treated with LPS (1 μg/ml) for 24h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MARCO antibody [EPR22944-64] ab239369).
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