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Rabbit Recombinant Monoclonal MARCO antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-MARCO antibody [EPR22944-66] (AB259264), expandable thumbnail
  • Western blot - Anti-MARCO antibody [EPR22944-66] (AB259264), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (AB259264), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (AB259264), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-66] (AB259264), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-FrIHC-P
Mouse
Not recommended
Not recommended
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed:7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MARCO antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22944-66
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264)

    Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. N-glycosylation is crucial for MARCO/Macrophage receptor with collagenous structure intercellular trafficking and surface targeting (PMID: 26892079).

    All lanes: Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264) at 1/1000 dilution

    Lane 1: Mouse spleen lysate at 20 µg

    Lane 2: Mouse spleen lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa, 75 kDa

    Exposure time: 3min

  • Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264)

    Blocking and Dilution Buffer and concentration: 5% NFDM/TBSTThe expression of MARCO/Macrophage receptor with collagenous structure is induced by Lipopolysaccharides (LPS) treatment (PMID: 9916718).

    All lanes: Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264) at 1/1000 dilution

    Lane 1: Untreated J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

    Lane 2: J774A.1 treated with 1ug/ml Lipopolysaccharides (LPS) for 24 hours, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 55-75 kDa

    Exposure time: 6s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (ab259264)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells of mouse liver. The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] (ab259264)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on macrophages of mouse spleen (PMID: 12874264, 15494482). The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-66] (ab259264)

    4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse spleen tissue labeling MARCO using ab259264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse splenic marginal zone (PMID: 24670793).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-66] (ab259264), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-66] (ab259264)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J744A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labelling MARCO with ab259264 at 1/50 (10 ug/ml) dilution, followed by ab259264 anti-MARCO Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). UNABLE TO ENCODE THE VARIABLE PLEASE DO MANUALLY is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor&~153;,594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab259264 anti-MARCO Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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