Rabbit Recombinant Monoclonal MARCO antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
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Mouse | Not recommended | Not recommended | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed:7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco
Rabbit Recombinant Monoclonal MARCO antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate. N-glycosylation is crucial for MARCO/Macrophage receptor with collagenous structure intercellular trafficking and surface targeting (PMID: 26892079).
All lanes: Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 20 µg
Lane 2: Mouse spleen lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa, 75 kDa
Exposure time: 3min
Blocking and Dilution Buffer and concentration: 5% NFDM/TBSTThe expression of MARCO/Macrophage receptor with collagenous structure is induced by Lipopolysaccharides (LPS) treatment (PMID: 9916718).
All lanes: Western blot - Anti-MARCO antibody [EPR22944-66] (ab259264) at 1/1000 dilution
Lane 1: Untreated J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg
Lane 2: J774A.1 treated with 1ug/ml Lipopolysaccharides (LPS) for 24 hours, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 55-75 kDa
Exposure time: 6s
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells of mouse liver. The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on macrophages of mouse spleen (PMID: 12874264, 15494482). The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse spleen tissue labeling MARCO using ab259264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse splenic marginal zone (PMID: 24670793).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J744A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labelling MARCO with ab259264 at 1/50 (10 ug/ml) dilution, followed by ab259264 anti-MARCO Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). UNABLE TO ENCODE THE VARIABLE PLEASE DO MANUALLY is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor&~153;,594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab259264 anti-MARCO Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
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