Rabbit Recombinant Monoclonal MARCO antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--flow-cytometry-img26713.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)")
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (AB271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--immunocytochemistry-immunofluorescence-img91694.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (AB271060)")
![Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--flow-cytometry-img66844.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed:7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
Alternative names
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco
Recommended products
Rabbit Recombinant Monoclonal MARCO antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24317-33
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--flow-cytometry-img26713.jpg "Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060)")
Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060)
Flow cytometric analysis of J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) treated with 500 ng/ml lipopolysaccharide (LPS) for 3 days (Red)/ Untreated control (Green) cells labelling MARCO with ab271060 at 1/500 dilution (0.1 μg) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (ab271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--immunocytochemistry-immunofluorescence-img91694.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (ab271060)")
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (ab271060)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 cells labelling MARCO with ab271060 at 1/500 (0.982 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing membranous and cytoplasmic staining in J774A.1 cell line treated with lipopolysaccharide (1 μg/ml) for 24 hours.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr24317-33-ab271060--flow-cytometry-img66844.jpg "Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060)")
Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (ab271060)
Flow cytometric analysis of mouse bone marrow cells treated with 10 ng/mL GM-CSF+5 ng/mL IL-4 for 24h (Right) / Untreated control (Left) cells labelling MARCO with ab271060 at 1/50 dilution (1 μg). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab271060, then stained with anti-CD11c conjugated to Alexa Fluor® 647 and MHC class II (I-A/I-E) conjugated to SB702.
Gated on viable CD11c positive population.
Downloads
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