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AB271060

Anti-MARCO antibody [EPR24317-33]

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(1 Publication)

Rabbit Recombinant Monoclonal MARCO antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (AB271060)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR24317-33] (AB271060)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 cells labelling MARCO with ab271060 at 1/500 (0.982 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in J774A.1 cell line treated with lipopolysaccharide (1 μg/ml) for 24 hours.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)

Flow cytometric analysis of mouse bone marrow cells treated with 10 ng/mL GM-CSF+5 ng/mL IL-4 for 24h (Right) / Untreated control (Left) cells labelling MARCO with ab271060 at 1/50 dilution (1 μg). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab271060, then stained with anti-CD11c conjugated to Alexa Fluor® 647 and MHC class II (I-A/I-E) conjugated to SB702.

Gated on viable CD11c positive population.

Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MARCO antibody [EPR24317-33] (AB271060)

Flow cytometric analysis of J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) treated with 500 ng/ml lipopolysaccharide (LPS) for 3 days (Red)/ Untreated control (Green) cells labelling MARCO with ab271060 at 1/500 dilution (0.1 μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

  • Carrier free

    Anti-MARCO antibody [EPR24317-33] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24317-33

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed : 7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
See full target information Marco
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature nanotechnology 19:1735-1744 PubMed39085390

2024

Polymersomes with splenic avidity target red pulp myeloid cells for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Annelies C Wauters,Jari F Scheerstra,Mandy M T van Leent,Abraham J P Teunissen,Bram Priem,Thijs J Beldman,Nils Rother,Raphaël Duivenvoorden,Geoffrey Prévot,Jazz Munitz,Yohana C Toner,Jeroen Deckers,Yuri van Elsas,Patricia Mora-Raimundo,Gal Chen,Sheqouia A Nauta,Anna Vera D Verschuur,Arjan W Griffioen,David P Schrijver,Tom Anbergen,Yudong Li,Hanglong Wu,Alexander F Mason,Marleen H M E van Stevendaal,Ewelina Kluza,Richard A J Post,Leo A B Joosten,Mihai G Netea,Claudia Calcagno,Zahi A Fayad,Roy van der Meel,Avi Schroeder,Loai K E A Abdelmohsen,Willem J M Mulder,Jan C M van Hest
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com