Rabbit Recombinant Monoclonal MARCO antibody. Carrier free. Suitable for mIHC, IHC-P, ICC/IF and reacts with Human samples.
Images
![Multiplex immunohistochemistry - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--multiplex-immunohistochemistry-img436024.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647)")
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunocytochemistry-immunofluorescence-img425231.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425211.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425210.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425209.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (AB314647)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IHC-P | WB | IP | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed:9468508). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (PubMed:12847263).
Alternative names
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2, MARCO, SCARA2
Recommended products
Rabbit Recombinant Monoclonal MARCO antibody. Carrier free. Suitable for mIHC, IHC-P, ICC/IF and reacts with Human samples.
Alternative names
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2, MARCO, SCARA2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26935-11
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab314647 is the carrier-free version of Anti-MARCO antibody [EPR26935-11] ab314646.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Multiplex immunohistochemistry - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--multiplex-immunohistochemistry-img436024.jpg "Multiplex immunohistochemistry - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)")
Multiplex immunohistochemistry - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)
This data was developed using Anti-MARCO antibody [EPR26935-11] ab314646, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue staining Prosurfactant Protein C with Anti-Prosurfactant Protein C antibody [EPR28174-18] ab312850 at a 1:2000 (0.26 ug/ml) dilution; Uteroglobin with Anti-Uteroglobin antibody [EPR27144-86] ab307666 at 1:2000 dilution and MARCO with Anti-MARCO antibody [EPR26935-11] ab314646 at 1:100 (5.1 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Prosurfactant Protein C (magenta; Opal™690), anti-Uteroglobin (green; Opal™520) and anti-MARCO (gray; Opal™570) on human lung.
Panel B: anti-Prosurfactant Protein C staining pneumocytes in human lung.
Panel C: anti-Uteroglobin staining club cells in human lung.
Panel D: anti-MARCO staining alveolar macrophages in human lung.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-Prosurfactant Protein C antibody [EPR28174-18] ab312850, Anti-Uteroglobin antibody [EPR27144-86] ab307666 and Anti-MARCO antibody [EPR26935-11] ab314646 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunocytochemistry-immunofluorescence-img425231.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)")
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)
This data was developed using Anti-MARCO antibody [EPR26935-11] ab314646, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling MARCO with Anti-MARCO antibody [EPR26935-11] ab314646 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membranous staining in THP-1 cells treated with PMA (80nM) for 72 hours.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425211.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)
This data was developed using Anti-MARCO antibody [EPR26935-11] ab314646, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MARCO with Anti-MARCO antibody [EPR26935-11] ab314646 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human pancreas (PMID: 34778091). The section was incubated with Anti-MARCO antibody [EPR26935-11] ab314646 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425210.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)
This data was developed using Anti-MARCO antibody [EPR26935-11] ab314646, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling MARCO with Anti-MARCO antibody [EPR26935-11] ab314646 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on macrophages of human lung (PMID: 10224290). The section was incubated with Anti-MARCO antibody [EPR26935-11] ab314646 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647), expandable thumbnail](https://content.abcam.com/products/images/marco-antibody-epr26935-11-bsa-and-azide-free-ab314647--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425209.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR26935-11] - BSA and Azide free (ab314647)
This data was developed using Anti-MARCO antibody [EPR26935-11] ab314646, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MARCO with Anti-MARCO antibody [EPR26935-11] ab314646 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on Kupffer cells of human liver (PMID: 27806438; PMID: 31381879). The section was incubated with Anti-MARCO antibody [EPR26935-11] ab314646 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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